Abstract
Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.
| Original language | English |
|---|---|
| Pages (from-to) | 271-276 |
| Number of pages | 6 |
| Journal | Sleep and Breathing |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 1 2016 |
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Keywords
- Electromyography (EMG)
- Human serotonin transporter
- Peripheral platelet
- Sleep bruxism
- Uptake ability
ASJC Scopus subject areas
- Otorhinolaryngology
- Clinical Neurology
Cite this
Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects. / Minakuchi, Hajime; Sogawa, Chiharu; Miki, Haruna; Hara, Emilio satoshi; Maekawa, Kenji; Sogawa, Norio; Kitayama, Shigeo; Matsuka, Yoshizo; Clark, Glenn Thomas; Kuboki, Takuo.
In: Sleep and Breathing, Vol. 20, No. 1, 01.03.2016, p. 271-276.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects
AU - Minakuchi, Hajime
AU - Sogawa, Chiharu
AU - Miki, Haruna
AU - Hara, Emilio satoshi
AU - Maekawa, Kenji
AU - Sogawa, Norio
AU - Kitayama, Shigeo
AU - Matsuka, Yoshizo
AU - Clark, Glenn Thomas
AU - Kuboki, Takuo
PY - 2016/3/1
Y1 - 2016/3/1
N2 - Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.
AB - Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.
KW - Electromyography (EMG)
KW - Human serotonin transporter
KW - Peripheral platelet
KW - Sleep bruxism
KW - Uptake ability
UR - http://www.scopus.com/inward/record.url?scp=84961126869&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961126869&partnerID=8YFLogxK
U2 - 10.1007/s11325-015-1281-0
DO - 10.1007/s11325-015-1281-0
M3 - Article
C2 - 26527205
AN - SCOPUS:84961126869
VL - 20
SP - 271
EP - 276
JO - Sleep and Breathing
JF - Sleep and Breathing
SN - 1520-9512
IS - 1
ER -