Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects

Hajime Minakuchi, Chiharu Sogawa, Haruna Miki, Emilio satoshi Hara, Kenji Maekawa, Norio Sogawa, Shigeo Kitayama, Yoshizo Matsuka, Glenn Thomas Clark, Takuo Kuboki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalSleep and Breathing
Volume20
Issue number1
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Sleep Bruxism
Serotonin Plasma Membrane Transport Proteins
Young Adult
Serotonin
Blood Platelets
Dental Schools
Aptitude
Phlebotomy
Electromyography
Orthodontics
Platelet Count
Antidepressive Agents
Fasting
Flow Cytometry
Smoking
Enzyme-Linked Immunosorbent Assay
Alcohols
Students

Keywords

  • Electromyography (EMG)
  • Human serotonin transporter
  • Peripheral platelet
  • Sleep bruxism
  • Uptake ability

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Clinical Neurology

Cite this

Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects. / Minakuchi, Hajime; Sogawa, Chiharu; Miki, Haruna; Hara, Emilio satoshi; Maekawa, Kenji; Sogawa, Norio; Kitayama, Shigeo; Matsuka, Yoshizo; Clark, Glenn Thomas; Kuboki, Takuo.

In: Sleep and Breathing, Vol. 20, No. 1, 01.03.2016, p. 271-276.

Research output: Contribution to journalArticle

@article{fd8aab6eeffe4f1c92bd5661c6a7e01a,
title = "Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects",
abstract = "Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.",
keywords = "Electromyography (EMG), Human serotonin transporter, Peripheral platelet, Sleep bruxism, Uptake ability",
author = "Hajime Minakuchi and Chiharu Sogawa and Haruna Miki and Emilio satoshi Hara and Kenji Maekawa and Norio Sogawa and Shigeo Kitayama and Yoshizo Matsuka and Clark, {Glenn Thomas} and Takuo Kuboki",
year = "2016",
month = "3",
day = "1",
doi = "10.1007/s11325-015-1281-0",
language = "English",
volume = "20",
pages = "271--276",
journal = "Sleep and Breathing",
issn = "1520-9512",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjects

AU - Minakuchi, Hajime

AU - Sogawa, Chiharu

AU - Miki, Haruna

AU - Hara, Emilio satoshi

AU - Maekawa, Kenji

AU - Sogawa, Norio

AU - Kitayama, Shigeo

AU - Matsuka, Yoshizo

AU - Clark, Glenn Thomas

AU - Kuboki, Takuo

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.

AB - Purpose: To evaluate correlations between serotonin transporter (SERT) uptake ability in human peripheral platelets and sleep bruxism (SB) frequency. Methods: Subjects were consecutively recruited from sixth-year students at Okayama University Dental School. Subjects were excluded if they (1) were receiving orthodontic treatment, (2) had a dermatological disease, (3) had taken an antidepressant within 6 months, or (4) had used an oral appliance within 6 months. SB frequency was determined as the summary score of three consecutive night assessments using a self-contained electromyography detector/analyzer in their home. Fasting peripheral venous blood samples were collected in the morning following the final SB assessment. SERT amount and platelet number were quantified via an ELISA assay and flow cytometry, respectively. Functional SERT characterization, 5-hydroxytryptamine (5-HT) uptake, maximum velocity (Vmax), and an affinity constant (Km) were assessed with a [3H] 5-HT uptake assay. The correlations between these variables and SB level were evaluated. Results: Among 50 eligible subjects (26 males, mean age 25.4 ± 2.41 years), 7 were excluded because of venipuncture failure, smoking, and alcohol intake during the experimental period. A small but significant negative correlation between SB level and [3H] 5-HT uptake was observed (Spearman’s correlation R2 = 0.063, p = 0.04). However, there were no significant correlations between SB level and total platelet amount, SERT, Vmax, and Km values (p = 0.08, 0.12, 0.71, and 0.68, respectively). Conclusions: Platelet serotonin uptake is significantly associated with SB frequency, yet only explains a small amount of SB variability.

KW - Electromyography (EMG)

KW - Human serotonin transporter

KW - Peripheral platelet

KW - Sleep bruxism

KW - Uptake ability

UR - http://www.scopus.com/inward/record.url?scp=84961126869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961126869&partnerID=8YFLogxK

U2 - 10.1007/s11325-015-1281-0

DO - 10.1007/s11325-015-1281-0

M3 - Article

C2 - 26527205

AN - SCOPUS:84961126869

VL - 20

SP - 271

EP - 276

JO - Sleep and Breathing

JF - Sleep and Breathing

SN - 1520-9512

IS - 1

ER -