SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis

Kenji Miura, Hiroyuki Okamoto, Eiji Okuma, Hayato Shiba, Hiroshi Kamada, Paul M. Hasegawa, Yoshiyuki Murata

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Transpiration and gas exchange occur through stomata. Thus, the control of stomatal aperture is important for the efficiency and regulation of water use, and for the response to drought. Here, we demonstrate that SIZ1-mediated endogenous salicylic acid (SA) accumulation plays an important role in stomatal closure and drought tolerance. siz1 reduced stomatal apertures. The reduced stomatal apertures of siz1 were inhibited by the application of peroxidase inhibitors, salicylhydroxamic acid and azide, which inhibits SA-dependent reactive oxygen species (ROS) production, but not by an NADPH oxidase inhibitor, diphenyl iodonium chloride, which inhibits ABA-dependent ROS production. Furthermore, the introduction of nahG into siz1, which reduces SA accumulation, restored stomatal opening. Stomatal closure is generally induced by water deficit. The siz1 mutation caused drought tolerance, whereas nahG siz1 suppressed the tolerant phenotype. Drought stresses also induced expression of SA-responsive genes, such as PR1 and PR2. Furthermore, other SA-accumulating mutants, cpr5 and acd6, exhibited stomatal closure and drought tolerance, and nahG suppressed the phenotype of cpr5 and acd6, as did siz1 and nahG siz1. Together, these results suggest that SIZ1 negatively affects stomatal closure and drought tolerance through the accumulation of SA.

Original languageEnglish
Pages (from-to)91-104
Number of pages14
JournalPlant Journal
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Salicylic Acid
Droughts
Arabidopsis
salicylic acid
drought tolerance
reactive oxygen species
Reactive Oxygen Species
Phenotype
phenotype
azides
Azides
Water
NADPH Oxidase
stomata
biphenyl
Peroxidase
gas exchange
Chlorides
transpiration
chlorides

Keywords

  • Drought tolerance
  • Guard cells
  • Reactive oxygen species
  • Salicylic acid
  • Stomatal closure
  • SUMO E3 ligase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis. / Miura, Kenji; Okamoto, Hiroyuki; Okuma, Eiji; Shiba, Hayato; Kamada, Hiroshi; Hasegawa, Paul M.; Murata, Yoshiyuki.

In: Plant Journal, Vol. 73, No. 1, 01.2013, p. 91-104.

Research output: Contribution to journalArticle

Miura, Kenji ; Okamoto, Hiroyuki ; Okuma, Eiji ; Shiba, Hayato ; Kamada, Hiroshi ; Hasegawa, Paul M. ; Murata, Yoshiyuki. / SIZ1 deficiency causes reduced stomatal aperture and enhanced drought tolerance via controlling salicylic acid-induced accumulation of reactive oxygen species in Arabidopsis. In: Plant Journal. 2013 ; Vol. 73, No. 1. pp. 91-104.
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