Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase

Akiya Akahoshi, Yoshitaka Suzue, Mizuki Kitamatsu, Masahiko Sisido, Takashi Ohtsuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Arginine analogs were incorporated site-specifically into proteins using an in vitro translation system. In this system, mRNAs containing a CGGG codon were translated by an aminoacyl-tRNA CCCG, which was charged with arginine analogs using yeast arginyl-tRNA synthetase. N G-monomethyl-l-arginine, l-citrulline and l-homoarginine were incorporated successfully into proteins using this method. The influence of arginine monomethylation in histone H3 on the acetylation of lysine residues by histone acetyltransferase hGCN5 was investigated, and the results demonstrated that K9 acetylation was suppressed by the methylation of R8 and R17 but not by R26 methylation. K18 acetylation was not affected by the methylation of R8, R17 and R26. This site-specific modification strategy provides a way to explore the roles of post-translational modifications in the absence of heterogeneity due to other modifications.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume414
Issue number3
DOIs
Publication statusPublished - Oct 28 2011

Fingerprint

Arginine-tRNA Ligase
Acetylation
Methylation
Arginine
Proteins
Homoarginine
Histone Acetyltransferases
Citrulline
Post Translational Protein Processing
Transfer RNA
Codon
Histones
Yeast
Lysine
Yeasts
Messenger RNA

Keywords

  • Arginine analog
  • Four-base codon
  • Histone H3
  • Methylarginine
  • Post-translational modification
  • TRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase. / Akahoshi, Akiya; Suzue, Yoshitaka; Kitamatsu, Mizuki; Sisido, Masahiko; Ohtsuki, Takashi.

In: Biochemical and Biophysical Research Communications, Vol. 414, No. 3, 28.10.2011, p. 625-630.

Research output: Contribution to journalArticle

Akahoshi, Akiya ; Suzue, Yoshitaka ; Kitamatsu, Mizuki ; Sisido, Masahiko ; Ohtsuki, Takashi. / Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 414, No. 3. pp. 625-630.
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