Singlet oxygen inhibits the repair of photosystem II by suppressing the translation elongation of the D1 protein in Synechocystis sp. PCC 6803

Yoshitaka Nishiyama, Suleyman Allakhverdiev, Hiroshi Yamamoto, Hidenori Hayashi, Norio Murata

Research output: Contribution to journalArticle

201 Citations (Scopus)

Abstract

Singlet oxygen, generated during photosynthesis, is a strong oxidant that can, potentially, damage various molecules of biological importance. We investigated the effects in vivo of singlet oxygen on the photodamage to photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. Increases in intracellular concentrations of singlet oxygen, caused by the presence of photosensitizers, such as rose bengal and ethyl eosin, stimulated the apparent photodamage to PSII. However, actual photodamage to PSII, as assessed in the presence of chloramphenicol, was unaffected by the production of singlet oxygen. These observations suggest that singlet oxygen produced by added photosensitizers acts by inhibiting the repair of photodamaged PSII. Labeling of proteins in vivo revealed that singlet oxygen inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern blotting analysis indicated that the accumulation of psbA mRNAs, which encode the D1 protein, was unaffected by the production of singlet oxygen. Subcellular localization of polysomes with bound psbA mRNAs suggested that the primary target of singlet oxygen might be the elongation step of translation.

Original languageEnglish
Pages (from-to)11321-11330
Number of pages10
JournalBiochemistry
Volume43
Issue number35
DOIs
Publication statusPublished - Sep 7 2004
Externally publishedYes

Fingerprint

Translational Peptide Chain Elongation
Synechocystis
Singlet Oxygen
Photosystem II Protein Complex
Elongation
Repair
Proteins
Photosensitizing Agents
Rose Bengal
Messenger RNA
Polyribosomes
Photosynthesis
Cyanobacteria
Chloramphenicol
Oxidants
Northern Blotting
Labeling

ASJC Scopus subject areas

  • Biochemistry

Cite this

Singlet oxygen inhibits the repair of photosystem II by suppressing the translation elongation of the D1 protein in Synechocystis sp. PCC 6803. / Nishiyama, Yoshitaka; Allakhverdiev, Suleyman; Yamamoto, Hiroshi; Hayashi, Hidenori; Murata, Norio.

In: Biochemistry, Vol. 43, No. 35, 07.09.2004, p. 11321-11330.

Research output: Contribution to journalArticle

Nishiyama, Yoshitaka ; Allakhverdiev, Suleyman ; Yamamoto, Hiroshi ; Hayashi, Hidenori ; Murata, Norio. / Singlet oxygen inhibits the repair of photosystem II by suppressing the translation elongation of the D1 protein in Synechocystis sp. PCC 6803. In: Biochemistry. 2004 ; Vol. 43, No. 35. pp. 11321-11330.
@article{a1c5e3613a2447239d95339770c46581,
title = "Singlet oxygen inhibits the repair of photosystem II by suppressing the translation elongation of the D1 protein in Synechocystis sp. PCC 6803",
abstract = "Singlet oxygen, generated during photosynthesis, is a strong oxidant that can, potentially, damage various molecules of biological importance. We investigated the effects in vivo of singlet oxygen on the photodamage to photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. Increases in intracellular concentrations of singlet oxygen, caused by the presence of photosensitizers, such as rose bengal and ethyl eosin, stimulated the apparent photodamage to PSII. However, actual photodamage to PSII, as assessed in the presence of chloramphenicol, was unaffected by the production of singlet oxygen. These observations suggest that singlet oxygen produced by added photosensitizers acts by inhibiting the repair of photodamaged PSII. Labeling of proteins in vivo revealed that singlet oxygen inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern blotting analysis indicated that the accumulation of psbA mRNAs, which encode the D1 protein, was unaffected by the production of singlet oxygen. Subcellular localization of polysomes with bound psbA mRNAs suggested that the primary target of singlet oxygen might be the elongation step of translation.",
author = "Yoshitaka Nishiyama and Suleyman Allakhverdiev and Hiroshi Yamamoto and Hidenori Hayashi and Norio Murata",
year = "2004",
month = "9",
day = "7",
doi = "10.1021/bi036178q",
language = "English",
volume = "43",
pages = "11321--11330",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "35",

}

TY - JOUR

T1 - Singlet oxygen inhibits the repair of photosystem II by suppressing the translation elongation of the D1 protein in Synechocystis sp. PCC 6803

AU - Nishiyama, Yoshitaka

AU - Allakhverdiev, Suleyman

AU - Yamamoto, Hiroshi

AU - Hayashi, Hidenori

AU - Murata, Norio

PY - 2004/9/7

Y1 - 2004/9/7

N2 - Singlet oxygen, generated during photosynthesis, is a strong oxidant that can, potentially, damage various molecules of biological importance. We investigated the effects in vivo of singlet oxygen on the photodamage to photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. Increases in intracellular concentrations of singlet oxygen, caused by the presence of photosensitizers, such as rose bengal and ethyl eosin, stimulated the apparent photodamage to PSII. However, actual photodamage to PSII, as assessed in the presence of chloramphenicol, was unaffected by the production of singlet oxygen. These observations suggest that singlet oxygen produced by added photosensitizers acts by inhibiting the repair of photodamaged PSII. Labeling of proteins in vivo revealed that singlet oxygen inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern blotting analysis indicated that the accumulation of psbA mRNAs, which encode the D1 protein, was unaffected by the production of singlet oxygen. Subcellular localization of polysomes with bound psbA mRNAs suggested that the primary target of singlet oxygen might be the elongation step of translation.

AB - Singlet oxygen, generated during photosynthesis, is a strong oxidant that can, potentially, damage various molecules of biological importance. We investigated the effects in vivo of singlet oxygen on the photodamage to photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. Increases in intracellular concentrations of singlet oxygen, caused by the presence of photosensitizers, such as rose bengal and ethyl eosin, stimulated the apparent photodamage to PSII. However, actual photodamage to PSII, as assessed in the presence of chloramphenicol, was unaffected by the production of singlet oxygen. These observations suggest that singlet oxygen produced by added photosensitizers acts by inhibiting the repair of photodamaged PSII. Labeling of proteins in vivo revealed that singlet oxygen inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern blotting analysis indicated that the accumulation of psbA mRNAs, which encode the D1 protein, was unaffected by the production of singlet oxygen. Subcellular localization of polysomes with bound psbA mRNAs suggested that the primary target of singlet oxygen might be the elongation step of translation.

UR - http://www.scopus.com/inward/record.url?scp=4444313478&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444313478&partnerID=8YFLogxK

U2 - 10.1021/bi036178q

DO - 10.1021/bi036178q

M3 - Article

C2 - 15366942

AN - SCOPUS:4444313478

VL - 43

SP - 11321

EP - 11330

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 35

ER -