Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.).

F. Soltani, Kenji Kato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Plastid markers are typically highly conserved, making them especially valuable for revealing phylogenetic relationship at or above the species level. Thirty five accessions of Cucumis melo L., belonging to the groups Flexuosus, Cantalupensis, Inodorus and Dudaim collected from western-north to western-south part of Iran analyzed was used for plastid single nucleotide polymorphism. A pair of dCAPS primer was used to estimate different nucleotide type of plastid subtype sequences. PCR amplification of the plastid DNA fragment containing rpl16 and rpl14 sequences was performed and products were digested with Apo? in 50°C thermal condition for 2 h. After digesting products subjected to electrophoresis on 2.5% agarose gel. Single nucleotide polymorphism of 35 accessions from various groups were detected by different size of band on gel and polymorphism at two loci were determined as A and T types. Results showed that all Flexuosus and Inodorus accessions revealed T-type plastid identity in loci. Two accessions of the Dudaim group and one accession of the Cantalupensis group classified in A-type group. Based on this investigation there was chloroplast genome variation between different types of melons in Iran and also plastid type distributed allopathically.

Original languageEnglish
Title of host publicationInternational Symposium on Biotechnology and Other Omics in Vegetable Science
PublisherInternational Society for Horticultural Science
Pages133-136
Number of pages4
Volume1145
ISBN (Electronic)9789462611344
DOIs
Publication statusPublished - Nov 8 2016

Publication series

NameActa Horticulturae
Volume1145
ISSN (Print)0567-7572

Fingerprint

Cucumis melo
melons
single nucleotide polymorphism
plastids
Iran
gels
loci
plastid DNA
agarose
electrophoresis
nucleotides
genetic polymorphism
heat
phylogeny

Keywords

  • A-type and T-type
  • Melon
  • Plastid sub-type
  • Single nucleotide polymorphism

ASJC Scopus subject areas

  • Horticulture

Cite this

Soltani, F., & Kato, K. (2016). Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.). In International Symposium on Biotechnology and Other Omics in Vegetable Science (Vol. 1145, pp. 133-136). (Acta Horticulturae; Vol. 1145). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2016.1145.21

Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.). / Soltani, F.; Kato, Kenji.

International Symposium on Biotechnology and Other Omics in Vegetable Science. Vol. 1145 International Society for Horticultural Science, 2016. p. 133-136 (Acta Horticulturae; Vol. 1145).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Soltani, F & Kato, K 2016, Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.). in International Symposium on Biotechnology and Other Omics in Vegetable Science. vol. 1145, Acta Horticulturae, vol. 1145, International Society for Horticultural Science, pp. 133-136. https://doi.org/10.17660/ActaHortic.2016.1145.21
Soltani F, Kato K. Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.). In International Symposium on Biotechnology and Other Omics in Vegetable Science. Vol. 1145. International Society for Horticultural Science. 2016. p. 133-136. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2016.1145.21
Soltani, F. ; Kato, Kenji. / Single nucleotide polymorphism analysis of plastid sub-type sequences of Iranian melon accessions (Cucumis melo L.). International Symposium on Biotechnology and Other Omics in Vegetable Science. Vol. 1145 International Society for Horticultural Science, 2016. pp. 133-136 (Acta Horticulturae).
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