Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence

Noriyuki Sugo, Masatoshi Morimatsu, Yoshiyuki Arai, Yoshinori Kousoku, Aya Ohkuni, Taishin Nomura, Toshio Yanagida, Nobuhiko Yamamoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Proper spatiotemporal gene expression is achieved by selective DNA binding of transcription factors in the genome. The most intriguing question is how dynamic interactions between transcription factors and their target sites contribute to gene regulation by recruiting the basal transcriptional machinery. Here we demonstrate individual binding and dissociation events of the transcription factor cAMP response element-binding protein (CREB), both in vitro and in living cells, using single-molecule imaging. Fluorescent-tagged CREB bound to its target sequence cAMP-response element (CRE) for a remarkably longer period (dissociation rate constant: 0.21s -1) than to an unrelated sequence (2.74s -1). Moreover, CREB resided at restricted positions in the living cell nucleus for a comparable period. These results suggest that CREB stimulates transcription by binding transiently to CRE in the time range of several seconds.

Original languageEnglish
Article number10662
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - Jun 3 2015
Externally publishedYes

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Cyclic AMP Response Element-Binding Protein
Transcription Factors
Response Elements
Cell Nucleus
Genome
Gene Expression
Single Molecule Imaging
DNA
Genes

ASJC Scopus subject areas

  • General

Cite this

Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence. / Sugo, Noriyuki; Morimatsu, Masatoshi; Arai, Yoshiyuki; Kousoku, Yoshinori; Ohkuni, Aya; Nomura, Taishin; Yanagida, Toshio; Yamamoto, Nobuhiko.

In: Scientific Reports, Vol. 5, 10662, 03.06.2015.

Research output: Contribution to journalArticle

Sugo, Noriyuki ; Morimatsu, Masatoshi ; Arai, Yoshiyuki ; Kousoku, Yoshinori ; Ohkuni, Aya ; Nomura, Taishin ; Yanagida, Toshio ; Yamamoto, Nobuhiko. / Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence. In: Scientific Reports. 2015 ; Vol. 5.
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