Single-molecule force spectroscopy reveals force-enhanced binding of calcium ions by gelsolin

Chunmei Lv, Xiang Gao, Wenfei Li, Bo Xue, Meng Qin, Leslie D. Burtnick, Hao Zhou, Yi Cao, Robert C. Robinson, Wei Wang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Force is increasingly recognized as an important element in controlling biological processes. Forces can deform native protein conformations leading to protein-specific effects. Protein-protein binding affinities may be decreased, or novel protein-protein interaction sites may be revealed, on mechanically stressing one or more components. Here we demonstrate that the calcium-binding affinity of the sixth domain of the actin-binding protein gelsolin (G6) can be enhanced by mechanical force. Our kinetic model suggests that the calcium-binding affinity of G6 increases exponentially with force, up to the point of G6 unfolding. This implies that gelsolin may be activated at lower calcium ion levels when subjected to tensile forces. The demonstration that cation-protein binding affinities can be force-dependent provides a new understanding of the complex behaviour of cation-regulated proteins in stressful cellular environments, such as those found in the cytoskeleton-rich leading edge and at cell adhesions.

Original languageEnglish
Article number4623
JournalNature communications
Volume5
DOIs
Publication statusPublished - Aug 7 2014
Externally publishedYes

Fingerprint

Gelsolin
calcium
Spectroscopy
Ions
Calcium
proteins
Molecules
spectroscopy
molecules
ions
Proteins
Protein Binding
affinity
Cations
Biological Phenomena
Microfilament Proteins
Protein Conformation
Cytoskeleton
Cell Adhesion
Cell adhesion

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Single-molecule force spectroscopy reveals force-enhanced binding of calcium ions by gelsolin. / Lv, Chunmei; Gao, Xiang; Li, Wenfei; Xue, Bo; Qin, Meng; Burtnick, Leslie D.; Zhou, Hao; Cao, Yi; Robinson, Robert C.; Wang, Wei.

In: Nature communications, Vol. 5, 4623, 07.08.2014.

Research output: Contribution to journalArticle

Lv, Chunmei ; Gao, Xiang ; Li, Wenfei ; Xue, Bo ; Qin, Meng ; Burtnick, Leslie D. ; Zhou, Hao ; Cao, Yi ; Robinson, Robert C. ; Wang, Wei. / Single-molecule force spectroscopy reveals force-enhanced binding of calcium ions by gelsolin. In: Nature communications. 2014 ; Vol. 5.
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