Abstract
Single channel currents of lysenin were measured using articial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNA/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were inuenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration- dependent with applied DNA. As the current uctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current uctuations.
| Original language | English |
|---|---|
| Pages (from-to) | 920-925 |
| Number of pages | 6 |
| Journal | Proceedings of the Japan Academy Series B: Physical and Biological Sciences |
| Volume | 86 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
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Keywords
- Current inhibition
- Hemolytic toxin
- Patch clamp
- Single channel current
ASJC Scopus subject areas
- General
Cite this
Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection. / Aoki, Takaaki; Hirano, Minako; Takeuchi, Yuko; Kobayashi, Toshihide; Yanagida, Toshio; Ide, Toru.
In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 86, No. 9, 2010, p. 920-925.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection
AU - Aoki, Takaaki
AU - Hirano, Minako
AU - Takeuchi, Yuko
AU - Kobayashi, Toshihide
AU - Yanagida, Toshio
AU - Ide, Toru
PY - 2010
Y1 - 2010
N2 - Single channel currents of lysenin were measured using articial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNA/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were inuenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration- dependent with applied DNA. As the current uctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current uctuations.
AB - Single channel currents of lysenin were measured using articial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNA/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were inuenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration- dependent with applied DNA. As the current uctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current uctuations.
KW - Current inhibition
KW - Hemolytic toxin
KW - Patch clamp
KW - Single channel current
UR - http://www.scopus.com/inward/record.url?scp=78549264764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78549264764&partnerID=8YFLogxK
U2 - 10.2183/pjab.86.920
DO - 10.2183/pjab.86.920
M3 - Article
C2 - 21084775
AN - SCOPUS:78549264764
VL - 86
SP - 920
EP - 925
JO - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
JF - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
SN - 0386-2208
IS - 9
ER -