Single channel properties of lysenin measured in artificial lipid bilayers and their applications to biomolecule detection

Takaaki Aoki, Minako Hirano, Yuko Takeuchi, Toshihide Kobayashi, Toshio Yanagida, Toru Ide

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Single channel currents of lysenin were measured using articial lipid bilayers formed on a glass micropipette tip. The single channel conductance for KCl, NaCl, CaCl2, and Trimethylammonium-Cl were 474 ± 87, 537 ± 66, 210 ± 14, and 274 ± 10 pS, respectively, while the permeability ratio PNA/PCl was 5.8. By adding poly(deoxy adenine) or poly(L-lysine) to one side of the bilayer, channel currents were inuenced when membrane voltages were applied to pass the charged molecules through the channel pores. Current inhibition process was concentration- dependent with applied DNA. As the current uctuations of α-hemolysin channels is often cited as the detector in a molecular sensor, these results suggest that by monitoring channel current changes, the lysenin channel has possibilities to detect interactions between it and certain biomolecules by its current uctuations.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume86
Issue number9
DOIs
Publication statusPublished - Nov 25 2010
Externally publishedYes

Keywords

  • Current inhibition
  • Hemolytic toxin
  • Patch clamp
  • Single channel current

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

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