Single- and double-walled carbon nanotubes enhance atherosclerogenesis by promoting monocyte adhesion to endothelial cells and endothelial progenitor cell dysfunction

Yuka Suzuki, Saeko Tada-Oikawa, Yasuhiko Hayashi, Kiyora Izuoka, Misa Kataoka, Shunsuke Ichikawa, Wenting Wu, Cai Zong, Gaku Ichihara, Sahoko Ichihara

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: The use of carbon nanotubes has increased lately. However, the cardiovascular effect of exposure to carbon nanotubes remains elusive. The present study investigated the effects of pulmonary exposure to single-walled carbon nanotubes (SWCNTs) and double-walled carbon nanotubes (DWCNTs) on atherosclerogenesis using normal human aortic endothelial cells (HAECs) and apolipoprotein E-deficient (ApoE-/-) mice, a model of human atherosclerosis. Methods: HAECs were cultured and exposed to SWCNTs or DWCNTs for 16 h. ApoE-/- mice were exposed to SWCNTs or DWCNTs (10 or 40 μg/mouse) once every other week for 10 weeks by pharyngeal aspiration. Results: Exposure to CNTs increased the expression level of adhesion molecule (ICAM-1) and enhanced THP-1 monocyte adhesion to HAECs. ApoE-/- mice exposed to CNTs showed increased plaque area in the aorta by oil red O staining and up-regulation of ICAM-1 expression in the aorta, compared with vehicle-treated ApoE-/- mice. Endothelial progenitor cells (EPCs) are mobilized from the bone marrow into the circulation and subsequently migrate to the site of endothelial damage and repair. Exposure of ApoE-/- mice to high-dose SWCNTs or DWCNTs reduced the colony-forming units of EPCs in the bone marrow and diminished their migration function. Conclusion: The results suggested that SWCNTs and DWCNTs enhanced atherosclerogenesis by promoting monocyte adhesion to endothelial cells and inducing EPC dysfunction.

Original languageEnglish
Article number54
JournalParticle and Fibre Toxicology
Volume13
Issue number1
DOIs
Publication statusPublished - Oct 13 2016

Keywords

  • Adhesion
  • Atherosclerosis
  • Carbon nanotubes
  • Endothelial cells
  • Endothelial progenitor cells

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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