Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo

Yosuke Okamoto, Wataru Sonoyama, Mitsuaki Ono, Kentaro Akiyama, Takuo Fujisawa, Masamitsu Oshima, Yohei Tsuchimoto, Yoshizo Matsuka, Tatsuji Yasuda, Songtao Shi, Takuo Kuboki

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Statin, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, is known to promote bone formation. However, it is not clear whether statin affects the differentiation of pulp cells. This study used a cell proliferation assay, cell cycle analysis, quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and in vivo transplantation to examine the effects of simvastatin on human dental pulp stem cells (DPSCs) in vitro and in vivo. Simvastatin at 1 μmol/L was able to significantly suppress the proliferation of DPSCs without inducing apoptosis. Quantitative RT-PCR revealed both osteocalcin and dentin sialophosphoprotein to be significantly up-regulated when DPSCs were cultured with simvastatin in comparison to bone morphogenetic protein-2 treatment. The in vivo transplantation data showed that simvastatin treatment promoted mineralized tissue formation. Taken together, these results suggest that statin might be an ideal active ingredient to accelerate the differentiation of DPSCs.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalJournal of Endodontics
Volume35
Issue number3
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Dental Pulp
Simvastatin
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Stem Cells
Reverse Transcriptase Polymerase Chain Reaction
Transplantation
Bone Morphogenetic Protein 2
Osteocalcin
Osteogenesis
Cell Differentiation
Cell Cycle
Oxidoreductases
Cell Proliferation
Apoptosis
In Vitro Techniques

Keywords

  • Cell differentiation
  • cell proliferation
  • dental pulp stem cells
  • dentin regeneration
  • pulp capping material
  • reparative dentin formation
  • simvastatin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo. / Okamoto, Yosuke; Sonoyama, Wataru; Ono, Mitsuaki; Akiyama, Kentaro; Fujisawa, Takuo; Oshima, Masamitsu; Tsuchimoto, Yohei; Matsuka, Yoshizo; Yasuda, Tatsuji; Shi, Songtao; Kuboki, Takuo.

In: Journal of Endodontics, Vol. 35, No. 3, 03.2009, p. 367-372.

Research output: Contribution to journalArticle

Okamoto, Y, Sonoyama, W, Ono, M, Akiyama, K, Fujisawa, T, Oshima, M, Tsuchimoto, Y, Matsuka, Y, Yasuda, T, Shi, S & Kuboki, T 2009, 'Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo', Journal of Endodontics, vol. 35, no. 3, pp. 367-372. https://doi.org/10.1016/j.joen.2008.11.024
Okamoto, Yosuke ; Sonoyama, Wataru ; Ono, Mitsuaki ; Akiyama, Kentaro ; Fujisawa, Takuo ; Oshima, Masamitsu ; Tsuchimoto, Yohei ; Matsuka, Yoshizo ; Yasuda, Tatsuji ; Shi, Songtao ; Kuboki, Takuo. / Simvastatin Induces the Odontogenic Differentiation of Human Dental Pulp Stem Cells In Vitro and In Vivo. In: Journal of Endodontics. 2009 ; Vol. 35, No. 3. pp. 367-372.
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