TY - JOUR
T1 - Simultaneous separation of 17 anions by capillary electrophoresis with the addition of an organic solvent
AU - Hata, Kazuki
AU - Nonaka, Noriko
AU - Sato, Nobuyuki
AU - Kaneta, Takashi
N1 - Publisher Copyright:
© 2021 Wiley-VCH GmbH.
PY - 2021/7
Y1 - 2021/7
N2 - Seventeen inorganic and organic anions, that normally are insufficiently separated via ion chromatography, were completely separated by the addition of an organic solvent to a solution of BGE combined with an adjustment of the apparent pH via CE in combination with indirect UV absorbance detection. Methanol, ethanol, and acetonitrile were examined for their utility in manipulating the selective separation of anions. Methanol and acetonitrile were better modifiers than ethanol at enhancing the resolution of anions comigrating in an aqueous solution of BGE. Methanol was selected as the modifier that provided the largest separation window that could achieve a complete separation of the target analytes. Via the use of methanol, manipulation of the selectivity between inorganic anions and that between inorganic and organic anions was enhanced, but the separation between organic anions remained difficult when only methanol was used. By varying the apparent pH of the BGE in the presence of 10% v/v methanol, however, the separation selectivity between organic anions was substantially improved. Eventually, 7 inorganic and 10 organic anions were simultaneously separated using BGE at a pH of 6.3 in the presence of 10% v/v methanol.
AB - Seventeen inorganic and organic anions, that normally are insufficiently separated via ion chromatography, were completely separated by the addition of an organic solvent to a solution of BGE combined with an adjustment of the apparent pH via CE in combination with indirect UV absorbance detection. Methanol, ethanol, and acetonitrile were examined for their utility in manipulating the selective separation of anions. Methanol and acetonitrile were better modifiers than ethanol at enhancing the resolution of anions comigrating in an aqueous solution of BGE. Methanol was selected as the modifier that provided the largest separation window that could achieve a complete separation of the target analytes. Via the use of methanol, manipulation of the selectivity between inorganic anions and that between inorganic and organic anions was enhanced, but the separation between organic anions remained difficult when only methanol was used. By varying the apparent pH of the BGE in the presence of 10% v/v methanol, however, the separation selectivity between organic anions was substantially improved. Eventually, 7 inorganic and 10 organic anions were simultaneously separated using BGE at a pH of 6.3 in the presence of 10% v/v methanol.
KW - Capillary electrophoresis
KW - Indirect absorbance detection
KW - Inorganic anion
KW - Organic anion
KW - Organic solvent
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U2 - 10.1002/elps.202100014
DO - 10.1002/elps.202100014
M3 - Article
C2 - 33724495
AN - SCOPUS:85103219271
VL - 42
SP - 1317
EP - 1322
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 12-13
ER -