Simultaneous quantitative analysis of prostaglandins and thromboxane after low-dose X irradiation

Kiyonori Yamaoka, Toru Obata, Keiji Iriyama, Toshiyasu Iwasaki, Yuko Hoshi

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Abstract

The appearance of prostaglandins and thromboxane in mouse serum after X irradiation was observed by simultaneous quantitative analysis using gas chromatography/mass spectrometry/selected ion monitoring with stable isotope dilution methods. Mice of two strains (C57BL/CN Jcl and BALB/c) showed similar responses to X irradiation. In C57BL/6N Jcl mice, 0.2 Gy irradiation elicited a significant increase in generation of prostanoids: Immediately after irradiation, the 6-keto PGF1 α:TXB2 ratio and the level of PGE2 increased, after 20 min 6-keto PGF1 α and PGE2 increased, and after 4 h PGE1 and PGE2 increased. In BALB/c mice, generation of prostanoids was increased significantly immediately after irradiation (6-keto PGF1 α, 6- keto PGF1 α:TXB2 ratio, PGF2), and the increase was maintained from 20 min to 4 h (PGE1, PGF2) after 0.2 Gy irradiation. In C57BL/6N Jcl mice, a significant increase in production of 9α,11β-PGF2 was observed at 20 min after irradiation. In BALB/c mice, a significant increase in 9α,11β-PGF2 was seen immediately after irradiation and was maintained for 20 min. In C57BL/6N Jcl mice, the level of 8-epi PGF2 α was clearly increased 4 h after 4 Gy irradiation. A slight and slow increase was also seen after 0.2 Gy irradiation. In BALB/c mice, 8-epi PGF2 α was increased significantly at 20 min and 4 h after 4 Gy irradiation. These results show that 0.2 Gy irradiation stimulates production of prostanoids related to the inflammatory response in mice.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalRadiation Research
Volume149
Issue number1
DOIs
Publication statusPublished - Jan 16 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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