Simultaneous optical and electrical single channel recordings on a PEG glass

Toru Ide, Yuko Takeuchi, Hiroyuki Noji, Kazuhito V. Tabata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Single molecule imaging of working ion-channels is much more difficult than that of water-soluble proteins because of the fragile nature of membranes and lateral diffusion of particles in the membranes, which does not allow fluorescent contamination for optical single channel recording. In this report, we reconstituted maxi-potassium channels from porcine uterine smooth muscle into artificial planar bilayers formed on poly(ethylene glycol) (PEG) modified glass and performed simultaneous optical and electrical recording of the single channels. The channels were immobilized in the membranes by anchoring to PEG molecules on the glass. The technique developed in this study should pave the way for single molecule pharmacology of ion-channels.

Original languageEnglish
Pages (from-to)8540-8543
Number of pages4
JournalLangmuir
Volume26
Issue number11
DOIs
Publication statusPublished - Jun 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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