Simultaneous Optical and Electrical Recording of Single Molecule Bonding to Single Channel Proteins

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two birds with one stone: A method is described for simultaneous optical and electrical recordings of single ion-channel proteins by combining the artificial bilayer technique and single-molecule imaging with a total internal reflection fluorescence microscope. Using this apparatus, interactions between single DNA molecules and single alpha-hemolysin channels are measured (see picture).

Original languageEnglish
Pages (from-to)3408-3411
Number of pages4
JournalChemPhysChem
Volume11
Issue number16
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Fingerprint

recording
proteins
Hemolysin Proteins
Molecules
Ion Channels
molecules
Proteins
Microscopes
deoxyribonucleic acid
Fluorescence
microscopes
Imaging techniques
fluorescence
DNA
interactions

Keywords

  • α-hemolysin
  • DNA
  • Ion channels
  • Single-channel recording
  • Single-molecule studies

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Atomic and Molecular Physics, and Optics

Cite this

Simultaneous Optical and Electrical Recording of Single Molecule Bonding to Single Channel Proteins. / Ide, Toru.

In: ChemPhysChem, Vol. 11, No. 16, 11.2010, p. 3408-3411.

Research output: Contribution to journalArticle

@article{b3b01e21cc1a4b83bcaa5229d39a4fe8,
title = "Simultaneous Optical and Electrical Recording of Single Molecule Bonding to Single Channel Proteins",
abstract = "Two birds with one stone: A method is described for simultaneous optical and electrical recordings of single ion-channel proteins by combining the artificial bilayer technique and single-molecule imaging with a total internal reflection fluorescence microscope. Using this apparatus, interactions between single DNA molecules and single alpha-hemolysin channels are measured (see picture).",
keywords = "α-hemolysin, DNA, Ion channels, Single-channel recording, Single-molecule studies",
author = "Toru Ide",
year = "2010",
month = "11",
doi = "10.1002/cphc.201000560",
language = "English",
volume = "11",
pages = "3408--3411",
journal = "ChemPhysChem",
issn = "1439-4235",
publisher = "Wiley-VCH Verlag",
number = "16",

}

TY - JOUR

T1 - Simultaneous Optical and Electrical Recording of Single Molecule Bonding to Single Channel Proteins

AU - Ide, Toru

PY - 2010/11

Y1 - 2010/11

N2 - Two birds with one stone: A method is described for simultaneous optical and electrical recordings of single ion-channel proteins by combining the artificial bilayer technique and single-molecule imaging with a total internal reflection fluorescence microscope. Using this apparatus, interactions between single DNA molecules and single alpha-hemolysin channels are measured (see picture).

AB - Two birds with one stone: A method is described for simultaneous optical and electrical recordings of single ion-channel proteins by combining the artificial bilayer technique and single-molecule imaging with a total internal reflection fluorescence microscope. Using this apparatus, interactions between single DNA molecules and single alpha-hemolysin channels are measured (see picture).

KW - α-hemolysin

KW - DNA

KW - Ion channels

KW - Single-channel recording

KW - Single-molecule studies

UR - http://www.scopus.com/inward/record.url?scp=78349232394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78349232394&partnerID=8YFLogxK

U2 - 10.1002/cphc.201000560

DO - 10.1002/cphc.201000560

M3 - Article

VL - 11

SP - 3408

EP - 3411

JO - ChemPhysChem

JF - ChemPhysChem

SN - 1439-4235

IS - 16

ER -