Simultaneous optical and electrical recording of a single ion-channel

Toru Ide, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In recent years, the single-molecule imaging technique has proven to be a valuable tool in solving many basic problems in biophysics. The technique used to measure single-molecule functions was initially developed to study electrophysiological properties of channel proteins. However, the technology to visualize single channels at work has not received as much attention. In this study, we have for the first time, simultaneously measured the optical and electrical properties of single-channel proteins. The large conductance calcium-activated potassium channel (BK-channel) labeled with fluorescent dye molecules was incorporated into a planar bilayer membrane and the fluorescent image captured with a total internal reflection fluorescence microscope simultaneously with single-channel current recording. This innovative technology will greatly advance the study of channel proteins as well as signal transduction processes that involve ion permeation processes.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalJapanese Journal of Physiology
Volume52
Issue number5
DOIs
Publication statusPublished - Oct 2002
Externally publishedYes

Fingerprint

Ion Channels
Large-Conductance Calcium-Activated Potassium Channels
Technology
Biophysics
Proteins
Fluorescent Dyes
Signal Transduction
Fluorescence
Ions
Membranes

Keywords

  • Bilayer
  • BK-channel
  • Imaging
  • Single-channel
  • Single-molecule

ASJC Scopus subject areas

  • Physiology

Cite this

Simultaneous optical and electrical recording of a single ion-channel. / Ide, Toru; Takeuchi, Yuko; Aoki, Takaaki; Yanagida, Toshio.

In: Japanese Journal of Physiology, Vol. 52, No. 5, 10.2002, p. 429-434.

Research output: Contribution to journalArticle

Ide, Toru ; Takeuchi, Yuko ; Aoki, Takaaki ; Yanagida, Toshio. / Simultaneous optical and electrical recording of a single ion-channel. In: Japanese Journal of Physiology. 2002 ; Vol. 52, No. 5. pp. 429-434.
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