TY - JOUR
T1 - Simultaneous measurement of monoamine metabolites and 5-methyltetrahydrofolate in the cerebrospinal fluid of children
AU - Akiyama, Tomoyuki
AU - Hayashi, Yumiko
AU - Hanaoka, Yoshiyuki
AU - Shibata, Takashi
AU - Akiyama, Mari
AU - Nakamura, Kazuyuki
AU - Tsuyusaki, Yu
AU - Kubota, Masaya
AU - Yoshinaga, Harumi
AU - Kobayashi, Katsuhiro
N1 - Funding Information:
This study was supported by the Japan Society for the Promotion of Science (Grant Number JP15K09622). This funding source had no involvement in the study design, the collection, analysis and interpretation of data, the writing of the report, or the decision to submit this article. We thank Dr. Shinpei Baba at Tokyo Medical and Dental University, Dr. Tetsuhiro Fukuyama at Nagano Children's Hospital, Dr. Shin-ichiro Hamano at Saitama Children's Medical Center, Dr. Ichiro Kuki at Osaka City General Hospital, Dr. Ken Momosaki at Kumamoto University, and Dr. Yoshihiro Toda at Tokushima University, for providing CSF samples from pediatric patients. We thank Eibunkousei.net (http://www.eibunkousei.net/) for English-language editing.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Background We describe a new method for simultaneous measurement of monoamine metabolites (3-O-methyldopa [3-OMD], 3-methoxy-4-hydroxyphenylethyleneglycol [MHPG], 5-hydroxyindoleacetic acid [5-HIAA], and homovanillic acid [HVA]) and 5-methyltetrahydrofolate (5-MTHF) and its use on cerebrospinal fluid (CSF) samples from pediatric patients. Methods Monoamine metabolites and 5-MTHF were measured by high-performance liquid chromatography with fluorescence detection. CSF samples were prospectively collected from children according to a standardized collection protocol in which the first 1-ml fraction was used for analysis. Results Monoamine metabolites and 5-MTHF were separated within 10 min. They showed linearity from the limit of detection to 1024 nmol/l. The limit of quantification of each metabolite was sufficiently low for the CSF sample assay. In 42 CSF samples after excluding cases with possibly altered neurotransmitter profiles, the concentrations of 3-OMD, MHPG, 5-HIAA, HVA, and 5-MTHF showed significant age dependence and their ranges were comparable with the reference values in the literature. The metabolite profiles of aromatic L-amino acid decarboxylase deficiency, Segawa disease, and folate receptor α defect by this method were compatible with those in the literature. Conclusions This method is a simple means of measuring CSF monoamine metabolites and 5-MTHF, and is especially useful for laboratories not equipped with electrochemical detectors.
AB - Background We describe a new method for simultaneous measurement of monoamine metabolites (3-O-methyldopa [3-OMD], 3-methoxy-4-hydroxyphenylethyleneglycol [MHPG], 5-hydroxyindoleacetic acid [5-HIAA], and homovanillic acid [HVA]) and 5-methyltetrahydrofolate (5-MTHF) and its use on cerebrospinal fluid (CSF) samples from pediatric patients. Methods Monoamine metabolites and 5-MTHF were measured by high-performance liquid chromatography with fluorescence detection. CSF samples were prospectively collected from children according to a standardized collection protocol in which the first 1-ml fraction was used for analysis. Results Monoamine metabolites and 5-MTHF were separated within 10 min. They showed linearity from the limit of detection to 1024 nmol/l. The limit of quantification of each metabolite was sufficiently low for the CSF sample assay. In 42 CSF samples after excluding cases with possibly altered neurotransmitter profiles, the concentrations of 3-OMD, MHPG, 5-HIAA, HVA, and 5-MTHF showed significant age dependence and their ranges were comparable with the reference values in the literature. The metabolite profiles of aromatic L-amino acid decarboxylase deficiency, Segawa disease, and folate receptor α defect by this method were compatible with those in the literature. Conclusions This method is a simple means of measuring CSF monoamine metabolites and 5-MTHF, and is especially useful for laboratories not equipped with electrochemical detectors.
KW - 3-O-methyldopa
KW - 3-methoxy-4-hydroxyphenylethyleneglycol
KW - 5-hydroxyindoleacetic acid
KW - 5-methyltetrahydrofolate
KW - Aromatic L-amino acid decarboxylase deficiency
KW - Cerebral folate deficiency
KW - Folate receptor α defect
KW - Homovanillic acid
KW - Neurotransmitters
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U2 - 10.1016/j.cca.2016.12.005
DO - 10.1016/j.cca.2016.12.005
M3 - Article
C2 - 27940130
AN - SCOPUS:85004140535
VL - 465
SP - 5
EP - 10
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
ER -