Abstract
Background: Methods for determining the origin of BK virus (BKV)-infected cells (decoy cells) in clinical urine samples have not been established although they could enhance the diagnosis of BKV infection in immunocompromised patients. Methods: We performed simultaneous immunostaining with anti-S100P (a urothelial marker) and anti-SV40 antibodies in 66 clinical urine samples exhibiting SV40 positivity and a decoy-cell appearance on Papanicolaou staining. The clinical voided urine samples included seven cases of renal transplantation, 47 cases of cancer therapy and 12 cases of non-neoplastic disease. SurePath™ liquid-based cytology was used for the urine samples. Results: BKV-infected cells were categorized as SV40(+)/S100P(+) and SV40 (+)/S100p(-). SV40(+)/S100P(-) cells were found in 55 cases (83.4%); nine cases (13.6%) carried both SV40(+)/S100P(-) and SV40(+)/S100P(+) cells. The former were identified as BKV infection in renal tubules and the latter in both the renal tubules and urothelial epithelia. The remaining two cases (3.0%) had only SV40(+)/S100P(+) cells of urothelial origin. Conclusion: Simultaneous immunostaining with anti-S100P and anti-SV40 is a useful method for determining the origin of BKV-infected cells in clinical urine samples from immunocompromised patients such as renal transplantation recipients.
| Original language | English |
|---|---|
| Pages (from-to) | 250-255 |
| Number of pages | 6 |
| Journal | Cytopathology |
| Volume | 26 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2015 |
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Keywords
- BK virus-infected cells
- S100P
- Simultaneous immunostaining
- SV40
- Urine cytology
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
Cite this
Simultaneous immunostaining with anti-S100P and anti-SV40 antibodies revealed the origin of BK virus-infected decoy cells in voided urine samples. / Ariyasu, S.; Yanai, Hiroyuki; Sato, M.; Shinno, Y.; Taniguchi, Kouhei; Yamadori, I.; Miki, Y.; Sato, Yasuharu; Yoshino, Tadashi; Takahashi, K.
In: Cytopathology, Vol. 26, No. 4, 01.08.2015, p. 250-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous immunostaining with anti-S100P and anti-SV40 antibodies revealed the origin of BK virus-infected decoy cells in voided urine samples
AU - Ariyasu, S.
AU - Yanai, Hiroyuki
AU - Sato, M.
AU - Shinno, Y.
AU - Taniguchi, Kouhei
AU - Yamadori, I.
AU - Miki, Y.
AU - Sato, Yasuharu
AU - Yoshino, Tadashi
AU - Takahashi, K.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Background: Methods for determining the origin of BK virus (BKV)-infected cells (decoy cells) in clinical urine samples have not been established although they could enhance the diagnosis of BKV infection in immunocompromised patients. Methods: We performed simultaneous immunostaining with anti-S100P (a urothelial marker) and anti-SV40 antibodies in 66 clinical urine samples exhibiting SV40 positivity and a decoy-cell appearance on Papanicolaou staining. The clinical voided urine samples included seven cases of renal transplantation, 47 cases of cancer therapy and 12 cases of non-neoplastic disease. SurePath™ liquid-based cytology was used for the urine samples. Results: BKV-infected cells were categorized as SV40(+)/S100P(+) and SV40 (+)/S100p(-). SV40(+)/S100P(-) cells were found in 55 cases (83.4%); nine cases (13.6%) carried both SV40(+)/S100P(-) and SV40(+)/S100P(+) cells. The former were identified as BKV infection in renal tubules and the latter in both the renal tubules and urothelial epithelia. The remaining two cases (3.0%) had only SV40(+)/S100P(+) cells of urothelial origin. Conclusion: Simultaneous immunostaining with anti-S100P and anti-SV40 is a useful method for determining the origin of BKV-infected cells in clinical urine samples from immunocompromised patients such as renal transplantation recipients.
AB - Background: Methods for determining the origin of BK virus (BKV)-infected cells (decoy cells) in clinical urine samples have not been established although they could enhance the diagnosis of BKV infection in immunocompromised patients. Methods: We performed simultaneous immunostaining with anti-S100P (a urothelial marker) and anti-SV40 antibodies in 66 clinical urine samples exhibiting SV40 positivity and a decoy-cell appearance on Papanicolaou staining. The clinical voided urine samples included seven cases of renal transplantation, 47 cases of cancer therapy and 12 cases of non-neoplastic disease. SurePath™ liquid-based cytology was used for the urine samples. Results: BKV-infected cells were categorized as SV40(+)/S100P(+) and SV40 (+)/S100p(-). SV40(+)/S100P(-) cells were found in 55 cases (83.4%); nine cases (13.6%) carried both SV40(+)/S100P(-) and SV40(+)/S100P(+) cells. The former were identified as BKV infection in renal tubules and the latter in both the renal tubules and urothelial epithelia. The remaining two cases (3.0%) had only SV40(+)/S100P(+) cells of urothelial origin. Conclusion: Simultaneous immunostaining with anti-S100P and anti-SV40 is a useful method for determining the origin of BKV-infected cells in clinical urine samples from immunocompromised patients such as renal transplantation recipients.
KW - BK virus-infected cells
KW - S100P
KW - Simultaneous immunostaining
KW - SV40
KW - Urine cytology
UR - http://www.scopus.com/inward/record.url?scp=84938091908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84938091908&partnerID=8YFLogxK
U2 - 10.1111/cyt.12213
DO - 10.1111/cyt.12213
M3 - Article
VL - 26
SP - 250
EP - 255
JO - Cytopathology
JF - Cytopathology
SN - 0956-5507
IS - 4
ER -