Simultaneous and rapid detection method for measles and rubella using single-tube multiplex real-time quantitative RT-PCR

Nori Yoshioka, Hideharu Hagiya, Matsuo Deguchi, Shigeto Hamaguchi, Masanori Kagita, Kazunori Tomono

Research output: Contribution to journalArticle

Abstract

Patients with measles or rubella infections manifest acute onset fever accompanying systemic exanthema, which are clinically difficult to be distinguish. Rapid diagnosis and differentiation of such epidemic viral diseases is essential to prevent outbreaks. We developed a single-tube multiplex real-time PCR assay for these indistinguishable viruses. We used previously-reported primer settings, with a slight modification of reporter dye, and applied to multiplex Taqman real-time PCR by cobas z480 (Roche Molecular Systems, Inc.). Consequently, the assay could detect 10 copies/10 μl of measles and rubella with coefficient of variations of 11.2% and 21.8%, respectively. Strengths of our methodology include simplicity of operation, short measurement time (2 h), uses of internal control (confirming a run of PCR), and quantitative measurement with high sensitivity. Both measles and rubella currently cause social outbreaks in Japan. We hope that our single-tube multiplex assay contributes to an early diagnosis, leading to an appropriate infection control measure and prevention of epidemics.

Original languageEnglish
Pages (from-to)829-831
Number of pages3
JournalJournal of Infection and Chemotherapy
Volume25
Issue number10
DOIs
Publication statusPublished - Oct 2019
Externally publishedYes

Fingerprint

Rubella
Measles
Real-Time Polymerase Chain Reaction
Disease Outbreaks
Multiplex Polymerase Chain Reaction
Virus Diseases
Infection Control
Exanthema
Early Diagnosis
Japan
Fever
Coloring Agents
Viruses
Polymerase Chain Reaction
Infection

Keywords

  • Infection control
  • Measles
  • Rapid diagnosis
  • Rubella

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Simultaneous and rapid detection method for measles and rubella using single-tube multiplex real-time quantitative RT-PCR. / Yoshioka, Nori; Hagiya, Hideharu; Deguchi, Matsuo; Hamaguchi, Shigeto; Kagita, Masanori; Tomono, Kazunori.

In: Journal of Infection and Chemotherapy, Vol. 25, No. 10, 10.2019, p. 829-831.

Research output: Contribution to journalArticle

Yoshioka, Nori ; Hagiya, Hideharu ; Deguchi, Matsuo ; Hamaguchi, Shigeto ; Kagita, Masanori ; Tomono, Kazunori. / Simultaneous and rapid detection method for measles and rubella using single-tube multiplex real-time quantitative RT-PCR. In: Journal of Infection and Chemotherapy. 2019 ; Vol. 25, No. 10. pp. 829-831.
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