Simple and sensitive headspace gas chromatographic determination of urinary methanol by means of syringe equilibration

Shigeru Suna; Nobuyuki Miyatake; Noriko Sakano; Takeshi Suzue; Fumiyuki Asakawa; Fumihiko Jitsunari; Tomohiro Hirao

doi:10.2116/bunsekikagaku.60.865

Simple and sensitive headspace gas chromatographic determination of urinary methanol by means of syringe equilibration

Shigeru Suna, Nobuyuki Miyatake, Noriko Sakano, Takeshi Suzue, Fumiyuki Asakawa, Fumihiko Jitsunari, Tomohiro Hirao

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.

Original language	English
Pages (from-to)	865-868
Number of pages	4
Journal	BUNSEKI KAGAKU
Volume	60
Issue number	11
DOIs	https://doi.org/10.2116/bunsekikagaku.60.865
Publication status	Published - 2011

Keywords

Biological
Headspace gas-chromatography
Syringe equilibration
Urinary methanol

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.",

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AU - Suna, Shigeru

AU - Miyatake, Nobuyuki

AU - Sakano, Noriko

AU - Suzue, Takeshi

AU - Asakawa, Fumiyuki

AU - Jitsunari, Fumihiko

AU - Hirao, Tomohiro

PY - 2011

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N2 - Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.

AB - Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.

KW - Biological

KW - Headspace gas-chromatography

KW - Syringe equilibration

KW - Urinary methanol

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