Abstract
Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.
Original language | English |
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Pages (from-to) | 865-868 |
Number of pages | 4 |
Journal | BUNSEKI KAGAKU |
Volume | 60 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2011 |
Keywords
- Biological
- Headspace gas-chromatography
- Syringe equilibration
- Urinary methanol
ASJC Scopus subject areas
- Analytical Chemistry