Abstract
Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 865-868 |
| Number of pages | 4 |
| Journal | Bunseki Kagaku |
| Volume | 60 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2011 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Simple and sensitive headspace gas chromatographic determination of urinary methanol by means of syringe equilibration. / Suna, Shigeru; Miyatake, Nobuyuki; Sakano, Noriko; Suzue, Takeshi; Asakawa, Fumiyuki; Jitsunari, Fumihiko; Hirao, Tomohiro.
In: Bunseki Kagaku, Vol. 60, No. 11, 2011, p. 865-868.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple and sensitive headspace gas chromatographic determination of urinary methanol by means of syringe equilibration
AU - Suna, Shigeru
AU - Miyatake, Nobuyuki
AU - Sakano, Noriko
AU - Suzue, Takeshi
AU - Asakawa, Fumiyuki
AU - Jitsunari, Fumihiko
AU - Hirao, Tomohiro
PY - 2011
Y1 - 2011
N2 - Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.
AB - Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.
KW - Biological
KW - Headspace gas-chromatography
KW - Syringe equilibration
KW - Urinary methanol
UR - http://www.scopus.com/inward/record.url?scp=83455200671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=83455200671&partnerID=8YFLogxK
U2 - 10.2116/bunsekikagaku.60.865
DO - 10.2116/bunsekikagaku.60.865
M3 - Article
AN - SCOPUS:83455200671
VL - 60
SP - 865
EP - 868
JO - Bunseki Kagaku
JF - Bunseki Kagaku
SN - 0525-1931
IS - 11
ER -