Simple and sensitive headspace gas chromatographic determination of urinary methanol by means of syringe equilibration

Shigeru Suna, Nobuyuki Miyatake, Noriko Sakano, Takeshi Suzue, Fumiyuki Asakawa, Fumihiko Jitsunari, Tomohiro Hirao

Research output: Contribution to journalArticle

Abstract

Simple and sensitive headspace gas-chromatographic (GC) analysis of urinary methanol using syringe equilibration method was developed. A glass syringe containing 30 μL of urine sample and 50 mg of glass wool was heated at 85 °C for 20 minutes and the HS vapor equilibrated under normal pressure in the syringe was injected directly into the GC-FID, and the analysis was carried out successfully without contamination. Relative standard deviations of less than 5 % were observed for 20-80 μg/mL methanol spiked urine samples. The quantitative detection limit for urinary methanol was 0.3 μg/mL.

Original languageEnglish
Pages (from-to)865-868
Number of pages4
JournalBUNSEKI KAGAKU
Volume60
Issue number11
DOIs
Publication statusPublished - Dec 19 2011

Keywords

  • Biological
  • Headspace gas-chromatography
  • Syringe equilibration
  • Urinary methanol

ASJC Scopus subject areas

  • Analytical Chemistry

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