Simple and rapid analysis of lamotrigine, a novel antiepileptic, in human serum by high-performance liquid chromatography using a solid-phase extraction technique

Syoichi Yamashita, Katsushi Furuno, Hiromu Kawasaki, Yutaka Gomita, Harumi Yoshinaga, Yasuko Yamatogi, Shunsuke Ohtahara

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Abstract

A simple and rapid method for the quantitation of concentrations of lamotrigine, a novel antiepileptic, in human serum was developed with high-performance liquid chromatography, using a solid-phase extraction technique. The mobile phase was composed of acetonitrile-10 mM phosphate buffer (pH 3.5) containing 5 mM sodium octanesulphonate (27:73, v/v), and components were detected at 265 nm. Retention times of acetanilide as an internal standard and lamotrigine were 3.4 and 10.3 min, respectively. The coefficients of variation were 3.1-4.5% and 4.4-9.8% for the within-day and between-day precision estimates, respectively. The extraction recovery of lamotrigine added to blank serum was 86-107%. The quantitation limit of lamotrigine was ca.0.2 μg/ml in 100 μl of serum. These results suggest that the method employed in this study is useful for the routine monitoring of sereum concentrations of lamotrigine in epileptic patients.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume670
Issue number2
DOIs
Publication statusPublished - Aug 18 1995

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ASJC Scopus subject areas

  • Chemistry(all)

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