Silicon accumulated in the shoots results in down-regulation of phosphorus transporter gene expression and decrease of phosphorus uptake in rice

An Yong Hu, Jing Che, Shao Jifeng, Kengo Yokosho, Xue Qiang Zhao, Ren Fang Shen, Jian Feng Ma

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aims: Silicon (Si) as a beneficial element can improve nutrient imbalance, but the molecular mechanism for this effect is poorly understood. The objective of this study is to examine the mechanism underlying Si-induced decrease of phosphorus (P) uptake in rice (Oryza sativa) at adequate/high P supply. Method: A rice mutant (lsi1) defective in Si uptake and its wild type (cv. Oochikara) were used. The P uptake was compared in the presence and absence of Si and the expression of Pi transporter genes was quantified. Results: Si addition in the nutrient solution significantly decreased shoot P concentration and uptake in the WT, but not in lsi1 mutant at two P levels, adequate (90 μM) and high (210 μM). Neither the root-to-shoot translocation of P nor the P distribution in different organs was altered by Si in both WT and lsi1. Heterogeneous expression of Lsi1 in Xenopus oocyte did not show transport activity for Pi. The expression of Pi transporter genes (OsPT1, 2 and 8) in the roots was hardly affected by Si in both WT and lsi1, but that of OsPT6 was down-regulated by Si in the WT roots, but not in the lsi1 roots. Furthermore, a split root experiment showed that Si accumulated in the shoots suppressed the expression of OsPT6. In rice grown in paddy field, lsi1 showed higher P concentration in the straw, husk and brown rice than the WT. Conclusion: Si decreased P uptake through down-regulating the expression of P transporter gene, OsPT6 in rice and Si accumulated in the shoot is required for this down-regulation.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPlant and Soil
DOIs
Publication statusAccepted/In press - Dec 5 2017

Fingerprint

silicon
gene expression
transporters
rice
shoot
phosphorus
uptake mechanisms
shoots
gene
regulation
mutants
brown rice
genes
nutrient
Xenopus
paddy field
hulls
translocation
straw
paddies

Keywords

  • Distribution
  • Down-regulation
  • P uptake
  • Rice
  • Silicon
  • Translocation
  • Transporter genes

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

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title = "Silicon accumulated in the shoots results in down-regulation of phosphorus transporter gene expression and decrease of phosphorus uptake in rice",
abstract = "Aims: Silicon (Si) as a beneficial element can improve nutrient imbalance, but the molecular mechanism for this effect is poorly understood. The objective of this study is to examine the mechanism underlying Si-induced decrease of phosphorus (P) uptake in rice (Oryza sativa) at adequate/high P supply. Method: A rice mutant (lsi1) defective in Si uptake and its wild type (cv. Oochikara) were used. The P uptake was compared in the presence and absence of Si and the expression of Pi transporter genes was quantified. Results: Si addition in the nutrient solution significantly decreased shoot P concentration and uptake in the WT, but not in lsi1 mutant at two P levels, adequate (90 μM) and high (210 μM). Neither the root-to-shoot translocation of P nor the P distribution in different organs was altered by Si in both WT and lsi1. Heterogeneous expression of Lsi1 in Xenopus oocyte did not show transport activity for Pi. The expression of Pi transporter genes (OsPT1, 2 and 8) in the roots was hardly affected by Si in both WT and lsi1, but that of OsPT6 was down-regulated by Si in the WT roots, but not in the lsi1 roots. Furthermore, a split root experiment showed that Si accumulated in the shoots suppressed the expression of OsPT6. In rice grown in paddy field, lsi1 showed higher P concentration in the straw, husk and brown rice than the WT. Conclusion: Si decreased P uptake through down-regulating the expression of P transporter gene, OsPT6 in rice and Si accumulated in the shoot is required for this down-regulation.",
keywords = "Distribution, Down-regulation, P uptake, Rice, Silicon, Translocation, Transporter genes",
author = "Hu, {An Yong} and Jing Che and Shao Jifeng and Kengo Yokosho and Zhao, {Xue Qiang} and Shen, {Ren Fang} and Ma, {Jian Feng}",
year = "2017",
month = "12",
day = "5",
doi = "10.1007/s11104-017-3512-6",
language = "English",
pages = "1--9",
journal = "Plant and Soil",
issn = "0032-079X",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Silicon accumulated in the shoots results in down-regulation of phosphorus transporter gene expression and decrease of phosphorus uptake in rice

AU - Hu, An Yong

AU - Che, Jing

AU - Jifeng, Shao

AU - Yokosho, Kengo

AU - Zhao, Xue Qiang

AU - Shen, Ren Fang

AU - Ma, Jian Feng

PY - 2017/12/5

Y1 - 2017/12/5

N2 - Aims: Silicon (Si) as a beneficial element can improve nutrient imbalance, but the molecular mechanism for this effect is poorly understood. The objective of this study is to examine the mechanism underlying Si-induced decrease of phosphorus (P) uptake in rice (Oryza sativa) at adequate/high P supply. Method: A rice mutant (lsi1) defective in Si uptake and its wild type (cv. Oochikara) were used. The P uptake was compared in the presence and absence of Si and the expression of Pi transporter genes was quantified. Results: Si addition in the nutrient solution significantly decreased shoot P concentration and uptake in the WT, but not in lsi1 mutant at two P levels, adequate (90 μM) and high (210 μM). Neither the root-to-shoot translocation of P nor the P distribution in different organs was altered by Si in both WT and lsi1. Heterogeneous expression of Lsi1 in Xenopus oocyte did not show transport activity for Pi. The expression of Pi transporter genes (OsPT1, 2 and 8) in the roots was hardly affected by Si in both WT and lsi1, but that of OsPT6 was down-regulated by Si in the WT roots, but not in the lsi1 roots. Furthermore, a split root experiment showed that Si accumulated in the shoots suppressed the expression of OsPT6. In rice grown in paddy field, lsi1 showed higher P concentration in the straw, husk and brown rice than the WT. Conclusion: Si decreased P uptake through down-regulating the expression of P transporter gene, OsPT6 in rice and Si accumulated in the shoot is required for this down-regulation.

AB - Aims: Silicon (Si) as a beneficial element can improve nutrient imbalance, but the molecular mechanism for this effect is poorly understood. The objective of this study is to examine the mechanism underlying Si-induced decrease of phosphorus (P) uptake in rice (Oryza sativa) at adequate/high P supply. Method: A rice mutant (lsi1) defective in Si uptake and its wild type (cv. Oochikara) were used. The P uptake was compared in the presence and absence of Si and the expression of Pi transporter genes was quantified. Results: Si addition in the nutrient solution significantly decreased shoot P concentration and uptake in the WT, but not in lsi1 mutant at two P levels, adequate (90 μM) and high (210 μM). Neither the root-to-shoot translocation of P nor the P distribution in different organs was altered by Si in both WT and lsi1. Heterogeneous expression of Lsi1 in Xenopus oocyte did not show transport activity for Pi. The expression of Pi transporter genes (OsPT1, 2 and 8) in the roots was hardly affected by Si in both WT and lsi1, but that of OsPT6 was down-regulated by Si in the WT roots, but not in the lsi1 roots. Furthermore, a split root experiment showed that Si accumulated in the shoots suppressed the expression of OsPT6. In rice grown in paddy field, lsi1 showed higher P concentration in the straw, husk and brown rice than the WT. Conclusion: Si decreased P uptake through down-regulating the expression of P transporter gene, OsPT6 in rice and Si accumulated in the shoot is required for this down-regulation.

KW - Distribution

KW - Down-regulation

KW - P uptake

KW - Rice

KW - Silicon

KW - Translocation

KW - Transporter genes

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U2 - 10.1007/s11104-017-3512-6

DO - 10.1007/s11104-017-3512-6

M3 - Article

SP - 1

EP - 9

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

ER -