Silencing of phosphoinositide dependent protein kinase orthologs reduces hypersensitive cell death in nicotiana benthamiana

Akinori Kiba, Kotoko Fukui, Maki Mitani, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Phosphatidic acid plays an important role in plant immune responses against phytopathogenic bacteria in Nicotiana benthamiana. Here we focused on phosphoinositide dependent protein kinases (PDKs) as a candidate required for phosphatidic acid signaling. Based on Arabidopsis PDK sequences, we identified four putative PDK orthologs in N. benthamiana genome. To address the role of PDKs in plant defense responses, we created all four NbPDKs-silenced plants by virus-induced gene silencing. the NbPDKs-silenced plants showed a moderately reduced growth phenotype. Induction of hypersensitive cell death was compromised in the NbPDKs-silenced plants challenged with Ralstonia solanacearum. The hypersensitive cell death induced by bacterial effectors was also reduced in the NbPDKs-silenced plants. the NbPDKssilenced plants showed decreased production of salicylic acid, jasmonic acid and jasmonoyl-L-isoleucine, as well as hydrogen peroxide after inoculation with R. solanacearum. These results suggest that NbPDKs might have an important role in the regulation of the hypersensitive cell death via plant hormone signaling and oxidative burst.

    Original languageEnglish
    Pages (from-to)363-367
    Number of pages5
    JournalPlant Biotechnology
    Volume37
    Issue number3
    DOIs
    Publication statusPublished - 2020

    Keywords

    • Effector-triggered immunity
    • Hypersensitive cell death
    • Nicotiana benthamiana
    • Phosphoinositide dependent protein kinase
    • Ralstonia solanacearum
    • Virus-induced gene silencing

    ASJC Scopus subject areas

    • Biotechnology
    • Agronomy and Crop Science
    • Plant Science

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