Silencing of Nicotiana benthamiana SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against Pseudomonas syringae

Akinori Kiba, Yu Imanaka, Masahito Nakano, Ivan Galis, Yuko Hojo, Tomonori Shinya, Kouhei Ohnishi, Yasufumi Hikichi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We previously identified SEC14, phospholipid transfer protein superfamily gene, in Nicotiana benthamiana (NbSEC14) that was closely related to phospholipid signaling as well as jasmonic acid-dependent defense responses during plant immune responses against Ralstonia solanacearum. To examine effect of NbSEC14-silencing on basal plant defenses, we used two other bacterial pathogens with different virulent strategies, Pseudomonas syringae pv. tabaci and pv. mellea. NbSEC14-silenced plants showed accelerated growth of P. syringae pv. tabaci and pv. mellea, and formation of necrotic lesions. Induction of JA-related PR-4 gene was compromised in NbSEC14-silenced plants, which was supported by reduced jasmonic acid levels in NbSEC14-silenced plants. These results suggested that NbSEC14 might be regulating plant basal resistance against plant pathogenic Pseudomonads via jasmonic acid-dependent signaling pathway.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalPlant Biotechnology
Volume33
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

Phospholipid Transfer Proteins
Pseudomonas syringae
Nicotiana benthamiana
jasmonic acid
Tobacco
Pseudomonas syringae pv. tabaci
Ralstonia solanacearum
Plant Immunity
lesions (plant)
Genes
plant response
lipid transfer proteins
Phospholipids
phospholipids
genes
immune response
pathogens
Growth

Keywords

  • Basal resistance
  • Jasmonic acid
  • Pseudomonas syringae
  • SEC14 phospholipid transfer protein
  • Virus-induced gene silencing

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Silencing of Nicotiana benthamiana SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against Pseudomonas syringae. / Kiba, Akinori; Imanaka, Yu; Nakano, Masahito; Galis, Ivan; Hojo, Yuko; Shinya, Tomonori; Ohnishi, Kouhei; Hikichi, Yasufumi.

In: Plant Biotechnology, Vol. 33, No. 2, 2016, p. 111-115.

Research output: Contribution to journalArticle

Kiba, Akinori ; Imanaka, Yu ; Nakano, Masahito ; Galis, Ivan ; Hojo, Yuko ; Shinya, Tomonori ; Ohnishi, Kouhei ; Hikichi, Yasufumi. / Silencing of Nicotiana benthamiana SEC14 phospholipid transfer protein reduced jasmonic acid dependent defense against Pseudomonas syringae. In: Plant Biotechnology. 2016 ; Vol. 33, No. 2. pp. 111-115.
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AU - Shinya, Tomonori

AU - Ohnishi, Kouhei

AU - Hikichi, Yasufumi

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AB - We previously identified SEC14, phospholipid transfer protein superfamily gene, in Nicotiana benthamiana (NbSEC14) that was closely related to phospholipid signaling as well as jasmonic acid-dependent defense responses during plant immune responses against Ralstonia solanacearum. To examine effect of NbSEC14-silencing on basal plant defenses, we used two other bacterial pathogens with different virulent strategies, Pseudomonas syringae pv. tabaci and pv. mellea. NbSEC14-silenced plants showed accelerated growth of P. syringae pv. tabaci and pv. mellea, and formation of necrotic lesions. Induction of JA-related PR-4 gene was compromised in NbSEC14-silenced plants, which was supported by reduced jasmonic acid levels in NbSEC14-silenced plants. These results suggested that NbSEC14 might be regulating plant basal resistance against plant pathogenic Pseudomonads via jasmonic acid-dependent signaling pathway.

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