TY - JOUR
T1 - Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl
AU - Ishii, Yuji
AU - Kato, Harutoshi
AU - Hatsumura, Megumu
AU - Ishida, Takumi
AU - Ariyoshi, Noritaka
AU - Oguri, Kazuta
N1 - Funding Information:
The authorst hank to Dr. Y. Ito in our faculty for kind cooperation of amino acid sequencing. This work was supportedi n part by a Grant-in-Aid for ScientificR esearchf rom the Ministry of Education, Science,S ports and Culture and the Ministry of Health and Welfare, Japan.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.
AB - A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.
KW - 3,3',4,4',5-Pentachlorobiphenyl (PCB 126)
KW - Aldolase, rat liver
KW - Amino acid sequence (peptide mapping)
KW - Gluconeogenesis, inhibition
KW - Polychlorinated biphenyl (PCB)
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U2 - 10.1016/S0300-483X(96)03543-3
DO - 10.1016/S0300-483X(96)03543-3
M3 - Article
C2 - 9020521
AN - SCOPUS:0031014867
VL - 116
SP - 193
EP - 199
JO - Toxicology
JF - Toxicology
SN - 0300-483X
IS - 1-3
ER -