Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl

Yuji Ishii; Harutoshi Kato; Megumu Hatsumura; Takumi Ishida; Noritaka Ariyoshi; Kazuta Oguri

doi:10.1016/S0300-483X(96)03543-3

Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl

Yuji Ishii, Harutoshi Kato, Megumu Hatsumura, Takumi Ishida, Noritaka Ariyoshi, Kazuta Oguri

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.

Original language	English
Pages (from-to)	193-199
Number of pages	7
Journal	Toxicology
Volume	116
Issue number	1-3
DOIs	https://doi.org/10.1016/S0300-483X(96)03543-3
Publication status	Published - Jan 15 1997
Externally published	Yes

Keywords

3,3',4,4',5-Pentachlorobiphenyl (PCB 126)
Aldolase, rat liver
Amino acid sequence (peptide mapping)
Gluconeogenesis, inhibition
Polychlorinated biphenyl (PCB)

ASJC Scopus subject areas

Toxicology

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10.1016/S0300-483X(96)03543-3

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title = "Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl",

abstract = "A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.",

keywords = "3,3',4,4',5-Pentachlorobiphenyl (PCB 126), Aldolase, rat liver, Amino acid sequence (peptide mapping), Gluconeogenesis, inhibition, Polychlorinated biphenyl (PCB)",

author = "Yuji Ishii and Harutoshi Kato and Megumu Hatsumura and Takumi Ishida and Noritaka Ariyoshi and Kazuta Oguri",

note = "Funding Information: The authorst hank to Dr. Y. Ito in our faculty for kind cooperation of amino acid sequencing. This work was supportedi n part by a Grant-in-Aid for ScientificR esearchf rom the Ministry of Education, Science,S ports and Culture and the Ministry of Health and Welfare, Japan.",

year = "1997",

month = jan,

day = "15",

doi = "10.1016/S0300-483X(96)03543-3",

language = "English",

volume = "116",

pages = "193--199",

journal = "Toxicology",

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T1 - Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl

AU - Ishii, Yuji

AU - Kato, Harutoshi

AU - Hatsumura, Megumu

AU - Ishida, Takumi

AU - Ariyoshi, Noritaka

AU - Oguri, Kazuta

N1 - Funding Information: The authorst hank to Dr. Y. Ito in our faculty for kind cooperation of amino acid sequencing. This work was supportedi n part by a Grant-in-Aid for ScientificR esearchf rom the Ministry of Education, Science,S ports and Culture and the Ministry of Health and Welfare, Japan.

PY - 1997/1/15

Y1 - 1997/1/15

N2 - A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.

AB - A toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl (PenCB), significantly suppresses the expression of liver aldolase B in rats. Hepatic aldolase activity in PenCB-treated rats was significantly reduced to about 50% of that in free- and pair-fed control groups. The reduced aldolase activity following PenCB-treatment was due to the marked suppression of the expression of aldolase B shown by immunoblot analysis after SDS-polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. The suppression of rat liver aldolase B could be a key biochemical lesion caused by PenCB.

KW - 3,3',4,4',5-Pentachlorobiphenyl (PCB 126)

KW - Aldolase, rat liver

KW - Amino acid sequence (peptide mapping)

KW - Gluconeogenesis, inhibition

KW - Polychlorinated biphenyl (PCB)

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U2 - 10.1016/S0300-483X(96)03543-3

DO - 10.1016/S0300-483X(96)03543-3

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JO - Toxicology

JF - Toxicology

SN - 0300-483X

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ER -

Significant suppression of rat liver aldolase B by a toxic coplanar polychlorinated biphenyl, 3,3',4,4',5-pentachlorobiphenyl

Abstract

Keywords

ASJC Scopus subject areas

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