A 54-kDa protein in rat liver cytosol was significantly induced by treatment with 3,4,5,3',4' -pentachlorobiphenyl (25 mg/kg, single i.p.) and 3-methylcholanthrene (20 mg/kg, once a day for 3 days, i.p.). The protein exhibited pI of 6.8 on two-dimensional gel electrophoresis. The amino acid sequences of peptide fragments from the protein digested in situ were highly similar to a selenium binding protein in mice and to the isoform acetaminophen binding protein in mice. The present result clearly demonstrates that a coplanar polychlorinated biphenyl and 3-methylcholanthrene are responsible for induction of selenium binding protein homologues. The physiological role of the mouse proteins, however, is not yet elucidated.
- 3,4,5,3',4'-Pentachlorobiphenyl (IUPAC PCB No. 126)
- Acetaminophen binding protein
- Selenium binding protein
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