Significant induction of a 54-kDa protein in rat liver with homologous alignment to mouse selenium binding protein by a coplanar polychlorinated biphenyl, 3,4,5,3',4'pentachlorobiphenyl and 3-methylcholanthrene

Yuji Ishii, Megumu Hatsumura, Takumi Ishida, Noritaka Ariyoshi, Kazuta Oguri

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20 Citations (Scopus)

Abstract

A 54-kDa protein in rat liver cytosol was significantly induced by treatment with 3,4,5,3',4' -pentachlorobiphenyl (25 mg/kg, single i.p.) and 3-methylcholanthrene (20 mg/kg, once a day for 3 days, i.p.). The protein exhibited pI of 6.8 on two-dimensional gel electrophoresis. The amino acid sequences of peptide fragments from the protein digested in situ were highly similar to a selenium binding protein in mice and to the isoform acetaminophen binding protein in mice. The present result clearly demonstrates that a coplanar polychlorinated biphenyl and 3-methylcholanthrene are responsible for induction of selenium binding protein homologues. The physiological role of the mouse proteins, however, is not yet elucidated.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalToxicology Letters
Volume87
Issue number1
DOIs
Publication statusPublished - Sep 1996
Externally publishedYes

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Keywords

  • 3,4,5,3',4'-Pentachlorobiphenyl (IUPAC PCB No. 126)
  • 3-Methylcholanthrene
  • Acetaminophen binding protein
  • Selenium
  • Selenium binding protein

ASJC Scopus subject areas

  • Toxicology

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