Abstract
We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
| Original language | English |
|---|---|
| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
| Pages | 1982-1984 |
| Number of pages | 3 |
| Volume | 3 |
| Publication status | Published - 2010 |
| Externally published | Yes |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: Oct 3 2010 → Oct 7 2010 |
Other
| Other | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Country | Netherlands |
| City | Groningen |
| Period | 10/3/10 → 10/7/10 |
Fingerprint
Keywords
- Carbonic anhydrase from bovine
- Entrapping liposome
- Guanidinium hydrochloride
- Lysozyme
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes. / Lorchirachoonkul, P.; Shimanouchi, Toshinori; Yamashita, K.; Umakoshi, H.; Kuboi, R.; Noda, M.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. p. 1982-1984.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes
AU - Lorchirachoonkul, P.
AU - Shimanouchi, Toshinori
AU - Yamashita, K.
AU - Umakoshi, H.
AU - Kuboi, R.
AU - Noda, M.
PY - 2010
Y1 - 2010
N2 - We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
AB - We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
KW - Carbonic anhydrase from bovine
KW - Entrapping liposome
KW - Guanidinium hydrochloride
KW - Lysozyme
UR - http://www.scopus.com/inward/record.url?scp=84884306186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884306186&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884306186
SN - 9781618390622
VL - 3
SP - 1982
EP - 1984
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ER -