Abstract
We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.
| Original language | English |
|---|---|
| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
| Pages | 1982-1984 |
| Number of pages | 3 |
| Publication status | Published - Dec 1 2010 |
| Externally published | Yes |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: Oct 3 2010 → Oct 7 2010 |
Publication series
| Name | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Volume | 3 |
Other
| Other | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Country | Netherlands |
| City | Groningen |
| Period | 10/3/10 → 10/7/10 |
Keywords
- Carbonic anhydrase from bovine
- Entrapping liposome
- Guanidinium hydrochloride
- Lysozyme
ASJC Scopus subject areas
- Control and Systems Engineering
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Cite this
Lorchirachoonkul, P., Shimanouchi, T., Yamashita, K., Umakoshi, H., Kuboi, R., & Noda, M. (2010). Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1982-1984). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 3).