Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes

P. Lorchirachoonkul, T. Shimanouchi, K. Yamashita, H. Umakoshi, R. Kuboi, M. Noda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a highly-sensitive leakage current microsensor by using DPPC liposome entrapping K4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads to obtain a prominent improvement although target protein, which is carbonic anhydrase from bovine (CAB) and lysozyme, weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB and lysozyme protein such as 'Native', 'Molten-Globule' and 'Unfold' states.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1982-1984
Number of pages3
Publication statusPublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Carbonic anhydrase from bovine
  • Entrapping liposome
  • Guanidinium hydrochloride
  • Lysozyme

ASJC Scopus subject areas

  • Control and Systems Engineering

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    Lorchirachoonkul, P., Shimanouchi, T., Yamashita, K., Umakoshi, H., Kuboi, R., & Noda, M. (2010). Significant improvement in sensitivity of leakage current microsensor by using denaturant and electrolyte-entrapping DPPC liposomes. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1982-1984). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 3).