Significance of proliferating cell nuclear antigen and argyrophilic nucleolar organizer regions in bone and soft-tissue spindle-cell tumors - PCNA-AgNORs index as a new parameter

Masahiko Kanamori, Hisao Matsui, Kazuo Ohmori, Yoshinobu Maeda, Shinichiro Tatezaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background. The grading of musculoskeletal spindle-cell tumors presents considerable problems. It is obvious that the prediction of the biological behavior in an individual may not always be possible when the conventional histological criteria (such as H and E/staining) are used. Methods. We investigated the expression of proliferating cell nuclear antigen (PCNA) and the number of argyrophilic nucleolar organizer regions (AgNORs) in paraffin sections of bone and soft tissue spindle-cell tumors from 41 patients. Results. The percentage of PCNA-positive cells per 600 nuclei was 35.4% in benign tumors (I); 38.8% in intermediate tumors (II); and 51.7% in malignant tumors (III) (II versus III, P < 0.05). (Tumors were graded as I, II, or III by histologic finding from H and E staining and according to clinical follow- up.) The average AgNORs score was 1.4 dots in (I); 1.9 dots in (II); and 3.0 dots in (III). There was a significant correlation between the AgNORs score and the histological grade (I versus II, P < 0.01: II versus III, P < 0.05: I versus III, P < 0.01). As a new parameter, a PCNA-AgNORs index plotted for individual patients afforded a cutoff value (1.0 dots · % /100) that proved to be useful for identifying histological grade, particularly for the differential diagnosis of intermediate and malignant tumors (I or II versus III, P < 0.001; I versus II, P < 0.05). Conclusion. The PCNA-AgNORs index is a good new parameter for assessing the histological grade of musculoskeletal spindle-cell tumors for the clinician and could be used as a routine examination.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalInternational Journal of Clinical Oncology
Volume4
Issue number2
DOIs
Publication statusPublished - Jan 1 1999
Externally publishedYes

Fingerprint

Nucleolus Organizer Region
Proliferating Cell Nuclear Antigen
Bone and Bones
Neoplasms
Staining and Labeling

Keywords

  • Argyrophilic nucleolar organizer regions (AgNORs)
  • Proliferating cell nuclear antigen (PCNA)
  • Spindle-cell tumors

ASJC Scopus subject areas

  • Surgery
  • Hematology
  • Oncology

Cite this

Significance of proliferating cell nuclear antigen and argyrophilic nucleolar organizer regions in bone and soft-tissue spindle-cell tumors - PCNA-AgNORs index as a new parameter. / Kanamori, Masahiko; Matsui, Hisao; Ohmori, Kazuo; Maeda, Yoshinobu; Tatezaki, Shinichiro.

In: International Journal of Clinical Oncology, Vol. 4, No. 2, 01.01.1999, p. 101-106.

Research output: Contribution to journalArticle

@article{5861068792eb4a24b07c58d5d3924a3d,
title = "Significance of proliferating cell nuclear antigen and argyrophilic nucleolar organizer regions in bone and soft-tissue spindle-cell tumors - PCNA-AgNORs index as a new parameter",
abstract = "Background. The grading of musculoskeletal spindle-cell tumors presents considerable problems. It is obvious that the prediction of the biological behavior in an individual may not always be possible when the conventional histological criteria (such as H and E/staining) are used. Methods. We investigated the expression of proliferating cell nuclear antigen (PCNA) and the number of argyrophilic nucleolar organizer regions (AgNORs) in paraffin sections of bone and soft tissue spindle-cell tumors from 41 patients. Results. The percentage of PCNA-positive cells per 600 nuclei was 35.4{\%} in benign tumors (I); 38.8{\%} in intermediate tumors (II); and 51.7{\%} in malignant tumors (III) (II versus III, P < 0.05). (Tumors were graded as I, II, or III by histologic finding from H and E staining and according to clinical follow- up.) The average AgNORs score was 1.4 dots in (I); 1.9 dots in (II); and 3.0 dots in (III). There was a significant correlation between the AgNORs score and the histological grade (I versus II, P < 0.01: II versus III, P < 0.05: I versus III, P < 0.01). As a new parameter, a PCNA-AgNORs index plotted for individual patients afforded a cutoff value (1.0 dots · {\%} /100) that proved to be useful for identifying histological grade, particularly for the differential diagnosis of intermediate and malignant tumors (I or II versus III, P < 0.001; I versus II, P < 0.05). Conclusion. The PCNA-AgNORs index is a good new parameter for assessing the histological grade of musculoskeletal spindle-cell tumors for the clinician and could be used as a routine examination.",
keywords = "Argyrophilic nucleolar organizer regions (AgNORs), Proliferating cell nuclear antigen (PCNA), Spindle-cell tumors",
author = "Masahiko Kanamori and Hisao Matsui and Kazuo Ohmori and Yoshinobu Maeda and Shinichiro Tatezaki",
year = "1999",
month = "1",
day = "1",
doi = "10.1007/s101470050035",
language = "English",
volume = "4",
pages = "101--106",
journal = "International Journal of Clinical Oncology",
issn = "1341-9625",
publisher = "Springer Japan",
number = "2",

}

TY - JOUR

T1 - Significance of proliferating cell nuclear antigen and argyrophilic nucleolar organizer regions in bone and soft-tissue spindle-cell tumors - PCNA-AgNORs index as a new parameter

AU - Kanamori, Masahiko

AU - Matsui, Hisao

AU - Ohmori, Kazuo

AU - Maeda, Yoshinobu

AU - Tatezaki, Shinichiro

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Background. The grading of musculoskeletal spindle-cell tumors presents considerable problems. It is obvious that the prediction of the biological behavior in an individual may not always be possible when the conventional histological criteria (such as H and E/staining) are used. Methods. We investigated the expression of proliferating cell nuclear antigen (PCNA) and the number of argyrophilic nucleolar organizer regions (AgNORs) in paraffin sections of bone and soft tissue spindle-cell tumors from 41 patients. Results. The percentage of PCNA-positive cells per 600 nuclei was 35.4% in benign tumors (I); 38.8% in intermediate tumors (II); and 51.7% in malignant tumors (III) (II versus III, P < 0.05). (Tumors were graded as I, II, or III by histologic finding from H and E staining and according to clinical follow- up.) The average AgNORs score was 1.4 dots in (I); 1.9 dots in (II); and 3.0 dots in (III). There was a significant correlation between the AgNORs score and the histological grade (I versus II, P < 0.01: II versus III, P < 0.05: I versus III, P < 0.01). As a new parameter, a PCNA-AgNORs index plotted for individual patients afforded a cutoff value (1.0 dots · % /100) that proved to be useful for identifying histological grade, particularly for the differential diagnosis of intermediate and malignant tumors (I or II versus III, P < 0.001; I versus II, P < 0.05). Conclusion. The PCNA-AgNORs index is a good new parameter for assessing the histological grade of musculoskeletal spindle-cell tumors for the clinician and could be used as a routine examination.

AB - Background. The grading of musculoskeletal spindle-cell tumors presents considerable problems. It is obvious that the prediction of the biological behavior in an individual may not always be possible when the conventional histological criteria (such as H and E/staining) are used. Methods. We investigated the expression of proliferating cell nuclear antigen (PCNA) and the number of argyrophilic nucleolar organizer regions (AgNORs) in paraffin sections of bone and soft tissue spindle-cell tumors from 41 patients. Results. The percentage of PCNA-positive cells per 600 nuclei was 35.4% in benign tumors (I); 38.8% in intermediate tumors (II); and 51.7% in malignant tumors (III) (II versus III, P < 0.05). (Tumors were graded as I, II, or III by histologic finding from H and E staining and according to clinical follow- up.) The average AgNORs score was 1.4 dots in (I); 1.9 dots in (II); and 3.0 dots in (III). There was a significant correlation between the AgNORs score and the histological grade (I versus II, P < 0.01: II versus III, P < 0.05: I versus III, P < 0.01). As a new parameter, a PCNA-AgNORs index plotted for individual patients afforded a cutoff value (1.0 dots · % /100) that proved to be useful for identifying histological grade, particularly for the differential diagnosis of intermediate and malignant tumors (I or II versus III, P < 0.001; I versus II, P < 0.05). Conclusion. The PCNA-AgNORs index is a good new parameter for assessing the histological grade of musculoskeletal spindle-cell tumors for the clinician and could be used as a routine examination.

KW - Argyrophilic nucleolar organizer regions (AgNORs)

KW - Proliferating cell nuclear antigen (PCNA)

KW - Spindle-cell tumors

UR - http://www.scopus.com/inward/record.url?scp=0033064898&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033064898&partnerID=8YFLogxK

U2 - 10.1007/s101470050035

DO - 10.1007/s101470050035

M3 - Article

AN - SCOPUS:0033064898

VL - 4

SP - 101

EP - 106

JO - International Journal of Clinical Oncology

JF - International Journal of Clinical Oncology

SN - 1341-9625

IS - 2

ER -