Abstract
The aim of this study was to determine the significance of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is known as a marker of oxidative stress in vivo, in patients with chronic renal failure (CRF). Fifty-one non-dialysed CRF patients (29 men and 22 women; mean ± SD age, 57.8 ± 12.8 years) who were under dietary therapy for at least 6 months were enrolled in the study. Both serum and urinary 8-OHdG levels were measured by using high-sensitive enzyme-linked immunosorbent assay (ELISA) kits. We examined the relationship between 8-OHdG levels and clinical indices in patients with CRF. As a result, the serum 8-OHdG level was strongly correlated with serum levels of urea nitrogen (UN; r = 0.58; P < 0.0001), creatinine (Cr; r = 0.53; P < 0.0001), and β2-microglobulin (β2-MG; r = 0.54; P < 0.0001). Furthermore, the serum 8-OHdG level was inversely correlated with creatinine clearance (Ccr; r = -0.54; P < 0.0001). In contrast, urinary 8-OHdG level was not correlated with any of the clinical parameters. This is the first report of 8-OHdG level determination in patients with CRF. It is suggested that serum 8-OHdG level is not sufficient as a marker of oxidative damage in patients with CRF, and it should be corrected according to the residual renal function to estimate the accurate degree of oxidative stress.
| Original language | English |
|---|---|
| Pages (from-to) | 192-195 |
| Number of pages | 4 |
| Journal | Nephrology |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1 2003 |
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Keywords
- 8-hydroxy-2′-deoxyguanosine
- Chronic renal failure
- Oxidative stress
ASJC Scopus subject areas
- Nephrology
Cite this
Significance of 8-hydroxy-2′-deoxyguanosine levels in patients with chronic renal failure. / Akagi, Shigeru; Nagake, Yoshio; Kasahara, Junko; Sarai, Ai; Kihara, Takashi; Morimoto, Hisanori; Yano, Ai; Nakao, Kazushi; Nanba, Kiyohito; Ichikawa, Haruo; Makino, Hirofumi.
In: Nephrology, Vol. 8, No. 4, 01.08.2003, p. 192-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Significance of 8-hydroxy-2′-deoxyguanosine levels in patients with chronic renal failure
AU - Akagi, Shigeru
AU - Nagake, Yoshio
AU - Kasahara, Junko
AU - Sarai, Ai
AU - Kihara, Takashi
AU - Morimoto, Hisanori
AU - Yano, Ai
AU - Nakao, Kazushi
AU - Nanba, Kiyohito
AU - Ichikawa, Haruo
AU - Makino, Hirofumi
PY - 2003/8/1
Y1 - 2003/8/1
N2 - The aim of this study was to determine the significance of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is known as a marker of oxidative stress in vivo, in patients with chronic renal failure (CRF). Fifty-one non-dialysed CRF patients (29 men and 22 women; mean ± SD age, 57.8 ± 12.8 years) who were under dietary therapy for at least 6 months were enrolled in the study. Both serum and urinary 8-OHdG levels were measured by using high-sensitive enzyme-linked immunosorbent assay (ELISA) kits. We examined the relationship between 8-OHdG levels and clinical indices in patients with CRF. As a result, the serum 8-OHdG level was strongly correlated with serum levels of urea nitrogen (UN; r = 0.58; P < 0.0001), creatinine (Cr; r = 0.53; P < 0.0001), and β2-microglobulin (β2-MG; r = 0.54; P < 0.0001). Furthermore, the serum 8-OHdG level was inversely correlated with creatinine clearance (Ccr; r = -0.54; P < 0.0001). In contrast, urinary 8-OHdG level was not correlated with any of the clinical parameters. This is the first report of 8-OHdG level determination in patients with CRF. It is suggested that serum 8-OHdG level is not sufficient as a marker of oxidative damage in patients with CRF, and it should be corrected according to the residual renal function to estimate the accurate degree of oxidative stress.
AB - The aim of this study was to determine the significance of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is known as a marker of oxidative stress in vivo, in patients with chronic renal failure (CRF). Fifty-one non-dialysed CRF patients (29 men and 22 women; mean ± SD age, 57.8 ± 12.8 years) who were under dietary therapy for at least 6 months were enrolled in the study. Both serum and urinary 8-OHdG levels were measured by using high-sensitive enzyme-linked immunosorbent assay (ELISA) kits. We examined the relationship between 8-OHdG levels and clinical indices in patients with CRF. As a result, the serum 8-OHdG level was strongly correlated with serum levels of urea nitrogen (UN; r = 0.58; P < 0.0001), creatinine (Cr; r = 0.53; P < 0.0001), and β2-microglobulin (β2-MG; r = 0.54; P < 0.0001). Furthermore, the serum 8-OHdG level was inversely correlated with creatinine clearance (Ccr; r = -0.54; P < 0.0001). In contrast, urinary 8-OHdG level was not correlated with any of the clinical parameters. This is the first report of 8-OHdG level determination in patients with CRF. It is suggested that serum 8-OHdG level is not sufficient as a marker of oxidative damage in patients with CRF, and it should be corrected according to the residual renal function to estimate the accurate degree of oxidative stress.
KW - 8-hydroxy-2′-deoxyguanosine
KW - Chronic renal failure
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=0042664090&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042664090&partnerID=8YFLogxK
U2 - 10.1046/j.1440-1797.2003.00163.x
DO - 10.1046/j.1440-1797.2003.00163.x
M3 - Article
C2 - 15012720
AN - SCOPUS:0042664090
VL - 8
SP - 192
EP - 195
JO - Nephrology
JF - Nephrology
SN - 1320-5358
IS - 4
ER -