Signal relay by CC chemokine receptor 2 (CCR2) and formylpeptide receptor 2 (Fpr2) in the recruitment of monocyte-derived dendritic cells in allergic airway inflammation

Keqiang Chen, Mingyong Liu, Ying Liu, Chunyan Wang, Teizo Yoshimura, Wanghua Gong, Yingying Le, Lino Tessarollo, Ji Ming Wang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Chemoattractant receptors regulate leukocyte accumulation at sites of inflammation. In allergic airway inflammation, although a chemokine receptor CCR2 was implicated in mediating monocyte-derived dendritic cell (DC) recruitment into the lung, we previously also discovered reduced accumulation of DCs in the inflamed lung in mice deficient in formylpeptide receptor Fpr2 (Fpr2-/-). We therefore investigated the role of Fpr2 in the trafficking of monocyte-derived DCs in allergic airway inflammation in cooperation with CCR2. We report that in allergic airway inflammation, CCR2 mediated the recruitment of monocyte-derived DCs to the perivascular region, and Fpr2 was required for further migration of the cells into the bronchiolar area. We additionally found that the bronchoalveolar lavage liquid from mice with airway inflammation contained both the CCR2 ligand CCL2 and an Fpr2 agonist CRAMP. Furthermore, similar to Fpr2-/- mice, in the inflamed airway of CRAMP-/- mice, DC trafficking into the peribronchiolar areas was diminished. Our study demonstrates that the interaction of CCR2 and Fpr2 with their endogenous ligands sequentially mediates the trafficking of DCs within the inflamed lung.

Original languageEnglish
Pages (from-to)16262-16273
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number23
DOIs
Publication statusPublished - Jun 7 2013
Externally publishedYes

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CCR2 Receptors
Dendritic Cells
Monocytes
Inflammation
Lung
Formyl Peptide Receptor
Ligands
Chemokine Receptors
Bronchoalveolar Lavage
Cell Movement
Leukocytes
Cells
Liquids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Signal relay by CC chemokine receptor 2 (CCR2) and formylpeptide receptor 2 (Fpr2) in the recruitment of monocyte-derived dendritic cells in allergic airway inflammation. / Chen, Keqiang; Liu, Mingyong; Liu, Ying; Wang, Chunyan; Yoshimura, Teizo; Gong, Wanghua; Le, Yingying; Tessarollo, Lino; Wang, Ji Ming.

In: Journal of Biological Chemistry, Vol. 288, No. 23, 07.06.2013, p. 16262-16273.

Research output: Contribution to journalArticle

Chen, Keqiang ; Liu, Mingyong ; Liu, Ying ; Wang, Chunyan ; Yoshimura, Teizo ; Gong, Wanghua ; Le, Yingying ; Tessarollo, Lino ; Wang, Ji Ming. / Signal relay by CC chemokine receptor 2 (CCR2) and formylpeptide receptor 2 (Fpr2) in the recruitment of monocyte-derived dendritic cells in allergic airway inflammation. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 23. pp. 16262-16273.
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