Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus

S. Yamamoto, N. Okujo, S. Matsuura, I. Fujiwara, Y. Fujita, S. Shinoda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Vibrio parahaemolyticus produces a structurally novel type of siderophore, termed vibrioferrin, in response to iron-limitation. This study was performed to examine whether vibrioferrin can assimilate iron from human iron-binding proteins for growth. Comparison of the growth rates between V. parahaemolyticus AQ 3354 and its spontaneously arising, vibrioferrin-deficient mutant revealed that vibrioferrin was able to sequester iron from 30% iron-saturated human transferrin for growth, but not from human lactoferrin even if fully saturated with iron. In both strains, iron limitation induced two high-molecular-weight outer membrane proteins with apparent molecular masses of approximately 78 and 83 kDa. Since only the outer membrane fraction including these proteins showed a binding capacity to ferric vibrioferrin complex, either of them may function as its cell surface receptor. These results suggested that the organism might utilize such a source of host iron through the action of vibrioferrin during in vivo survival and proliferation, although its importance in pathogenesis is unknown.

Original languageEnglish
Pages (from-to)687-693
Number of pages7
JournalMicrobiology and Immunology
Volume38
Issue number9
Publication statusPublished - 1994

Fingerprint

Vibrio parahaemolyticus
Siderophores
Transferrin
Iron
Growth
Iron-Binding Proteins
Lactoferrin
Cell Surface Receptors
vibrioferrin
Membrane Proteins
Molecular Weight
Membranes
Survival

Keywords

  • Iron source
  • Transferrin
  • Vibrio parahaemolyticus
  • Vibrioferrin

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Yamamoto, S., Okujo, N., Matsuura, S., Fujiwara, I., Fujita, Y., & Shinoda, S. (1994). Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus. Microbiology and Immunology, 38(9), 687-693.

Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus. / Yamamoto, S.; Okujo, N.; Matsuura, S.; Fujiwara, I.; Fujita, Y.; Shinoda, S.

In: Microbiology and Immunology, Vol. 38, No. 9, 1994, p. 687-693.

Research output: Contribution to journalArticle

Yamamoto, S, Okujo, N, Matsuura, S, Fujiwara, I, Fujita, Y & Shinoda, S 1994, 'Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus', Microbiology and Immunology, vol. 38, no. 9, pp. 687-693.
Yamamoto S, Okujo N, Matsuura S, Fujiwara I, Fujita Y, Shinoda S. Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus. Microbiology and Immunology. 1994;38(9):687-693.
Yamamoto, S. ; Okujo, N. ; Matsuura, S. ; Fujiwara, I. ; Fujita, Y. ; Shinoda, S. / Siderophore-mediated utilization of iron bound to transferrin by vibrio parahaemolyticus. In: Microbiology and Immunology. 1994 ; Vol. 38, No. 9. pp. 687-693.
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