Shotgun cloning and characterization of the thymidylate synthase- encoding gene from Mycobacterium bovis BCG

Sohkichi Matsumoto, Hideharu Yukitake, Naoya Ohara, Tohru Dairi, Hiroji Kanbara, Takeshi Yamada

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalMICROBIOLOGY and IMMUNOLOGY
Volume42
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Gene expression
  • Mycobacterial enzyme
  • ThyA

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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