Short panicle1 encodes a putative PTR family transporter and determines rice panicle size

Shengben Li, Qian Qian, Zhiming Fu, Dali Zeng, Xiangbing Meng, Junko Kyozuka, Masahiko Maekawa, Xudong Zhu, Jian Zhang, Jiayang Li, Yonghong Wang

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Summary The architecture of the rice inflorescence, which is determined mainly by the number and length of primary and secondary inflorescence branches, is of importance in both agronomy and developmental biology. The position and number of primary branches are established during the phase transition from vegetative to reproductive growth, and several of the genes identified as participating in this process do so by regulating the meristemic activities of inflorescence. However, little is known about the molecular mechanism that controls inflorescence branch elongation. Here, we report on a novel rice mutant, short panicle1 (sp1), which is defective in rice panicle elongation, and thus leads to the short-panicle phenotype. Gene cloning and characterization indicate that SP1 encodes a putative transporter that belongs to the peptide transporter (PTR) family. This conclusion is based on the findings that SP1 contains a conserved PTR2 domain consisting of 12 transmembrane domains, and that the SP1-GFP fusion protein is localized in the plasma membrane. The SP1 gene is highly expressed in the phloem of the branches of young panicles, which is consistent with the predicted function of SP1 and the sp1 phenotype. Phylogenetic analysis implies that SP1 might be a nitrate transporter. However, neither nitrate transporter activity nor any other compounds transported by known PTR proteins could be detected in either a Xenopus oocyte or yeast system, in our study, suggesting that SP1 may need other component(s) to be able to function as a transporter, or that it transports unknown substrates in the monocotyledonous rice plant.

Original languageEnglish
Pages (from-to)592-605
Number of pages14
JournalPlant Journal
Volume58
Issue number4
DOIs
Publication statusPublished - May 2009

Fingerprint

peptide transporters
Inflorescence
transporters
inflorescences
rice
Genes
Phenotype
Phloem
Developmental Biology
nitrates
Phase Transition
phenotype
Xenopus
agronomy
Oocytes
Organism Cloning
phase transition
Proteins
Yeasts
phloem

Keywords

  • Branch elongation
  • Oryza sativa L
  • Panicle
  • Peptide transporter family
  • Short panicle1

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Li, S., Qian, Q., Fu, Z., Zeng, D., Meng, X., Kyozuka, J., ... Wang, Y. (2009). Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. Plant Journal, 58(4), 592-605. https://doi.org/10.1111/j.1365-313X.2009.03799.x

Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. / Li, Shengben; Qian, Qian; Fu, Zhiming; Zeng, Dali; Meng, Xiangbing; Kyozuka, Junko; Maekawa, Masahiko; Zhu, Xudong; Zhang, Jian; Li, Jiayang; Wang, Yonghong.

In: Plant Journal, Vol. 58, No. 4, 05.2009, p. 592-605.

Research output: Contribution to journalArticle

Li, S, Qian, Q, Fu, Z, Zeng, D, Meng, X, Kyozuka, J, Maekawa, M, Zhu, X, Zhang, J, Li, J & Wang, Y 2009, 'Short panicle1 encodes a putative PTR family transporter and determines rice panicle size', Plant Journal, vol. 58, no. 4, pp. 592-605. https://doi.org/10.1111/j.1365-313X.2009.03799.x
Li, Shengben ; Qian, Qian ; Fu, Zhiming ; Zeng, Dali ; Meng, Xiangbing ; Kyozuka, Junko ; Maekawa, Masahiko ; Zhu, Xudong ; Zhang, Jian ; Li, Jiayang ; Wang, Yonghong. / Short panicle1 encodes a putative PTR family transporter and determines rice panicle size. In: Plant Journal. 2009 ; Vol. 58, No. 4. pp. 592-605.
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AU - Kyozuka, Junko

AU - Maekawa, Masahiko

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AU - Zhang, Jian

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AU - Wang, Yonghong

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