Short Hydrogen bond between redox-active tyrosine Y Z and D1-His190 in the photosystem II crystal structure

Keisuke Saito, Jian-Ren Shen, Toyokazu Ishida, Hiroshi Ishikita

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The crystal structure of photosystem II (PSII) analyzed at a resolution of 1.9 Å revealed a remarkably short H-bond between redox-active tyrosine Y Z and D1-His190 (2.46 Å donor-acceptor distance). Using large-scale quantum mechanical/molecular mechanical (QM/MM) calculations with the explicit PSII protein environment, we were able to reproduce this remarkably short H-bond in the original geometry of the crystal structure in the neutral [Y ZO•••H•••N ε-His- N δH•••O=Asn] state, but not in the oxidized states, indicating that the neutral state was the one observed in the crystal structure. In addition to the appropriate redox/protonation state of Y Z and D1-His190, we found that the presence of a cluster of water molecules played a key role in shortening the distance between Y Z and D1-His190. The orientations of the water molecules in the cluster were energetically stabilized by the highly polarized PSII protein environment, where the Ca ion of the oxygen-evolving complex (OEC) and the OEC ligand D1-Glu189 were also involved.

Original languageEnglish
Pages (from-to)9836-9844
Number of pages9
JournalBiochemistry
Volume50
Issue number45
DOIs
Publication statusPublished - Nov 15 2011

Fingerprint

Photosystem II Protein Complex
Oxidation-Reduction
Tyrosine
Hydrogen
Hydrogen bonds
Crystal structure
Oxygen
Molecules
Water
Protonation
Proteins
Ions
Ligands
Geometry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Short Hydrogen bond between redox-active tyrosine Y Z and D1-His190 in the photosystem II crystal structure. / Saito, Keisuke; Shen, Jian-Ren; Ishida, Toyokazu; Ishikita, Hiroshi.

In: Biochemistry, Vol. 50, No. 45, 15.11.2011, p. 9836-9844.

Research output: Contribution to journalArticle

Saito, Keisuke ; Shen, Jian-Ren ; Ishida, Toyokazu ; Ishikita, Hiroshi. / Short Hydrogen bond between redox-active tyrosine Y Z and D1-His190 in the photosystem II crystal structure. In: Biochemistry. 2011 ; Vol. 50, No. 45. pp. 9836-9844.
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