Short germ insects utilize both the ancestral and derived mode of polycomb group-mediated epigenetic silencing of hox genes

Yuji Matsuoka, Tetsuya Bando, Takahito Watanabe, Yoshiyasu Ishimaru, Sumihare Noji, Aleksandar Popadić, Taro Mito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In insect species that undergo long germ segmentation, such as Drosophila, all segments are specified simultaneously at the early blastoderm stage. As embryogenesis progresses, the expression boundaries of Hox genes are established by repression of gap genes, which is subsequently replaced by Polycomb group (PcG) silencing. At present, however, it is not known whether patterning occurs this way in a more ancestral (short germ) mode of embryogenesis, where segments are added gradually during posterior elongation. In this study, two members of the PcG family, Enhancer of zeste (E(z)) and Suppressor of zeste 12 (Su(z)12), were analyzed in the short germ cricket, Gryllus bimaculatus. Results suggest that although stepwise negative regulation by gap and PcG genes is present in anterior members of the Hox cluster, it does not account for regulation of two posterior Hox genes, abdominal-A (abd-A) and Abdominal-B (Abd-B). Instead, abd-A and Abd-B are predominantly regulated by PcG genes, which is the mode present in vertebrates. These findings suggest that an intriguing transition of the PcG-mediated silencing of Hox genes may have occurred during animal evolution. The ancestral bilaterian state may have resembled the current vertebrate mode of regulation, where PcG-mediated silencing of Hox genes occurs before their expression is initiated and is responsible for the establishment of individual expression domains. Then, during insect evolution, the repression by transcription factors may have been acquired in anterior Hox genes of short germ insects, while PcG silencing was maintained in posterior Hox genes.

Original languageEnglish
Pages (from-to)702-709
Number of pages8
JournalBiology Open
Volume4
Issue number6
DOIs
Publication statusPublished - Jun 15 2015

Fingerprint

Homeobox Genes
gene silencing
Epigenomics
epigenetics
Insects
Genes
insects
genes
Embryonic Development
Vertebrates
Blastoderm
embryogenesis
Gryllidae
vertebrates
blastoderm
Gryllus bimaculatus
Drosophila
Transcription Factors
transcription factors
Elongation

Keywords

  • Epigenetic silencing
  • Gene expression
  • Hox genes
  • Insec
  • Polycomb group genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Short germ insects utilize both the ancestral and derived mode of polycomb group-mediated epigenetic silencing of hox genes. / Matsuoka, Yuji; Bando, Tetsuya; Watanabe, Takahito; Ishimaru, Yoshiyasu; Noji, Sumihare; Popadić, Aleksandar; Mito, Taro.

In: Biology Open, Vol. 4, No. 6, 15.06.2015, p. 702-709.

Research output: Contribution to journalArticle

Matsuoka, Yuji ; Bando, Tetsuya ; Watanabe, Takahito ; Ishimaru, Yoshiyasu ; Noji, Sumihare ; Popadić, Aleksandar ; Mito, Taro. / Short germ insects utilize both the ancestral and derived mode of polycomb group-mediated epigenetic silencing of hox genes. In: Biology Open. 2015 ; Vol. 4, No. 6. pp. 702-709.
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