Short communication: Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation

Mitsuyuki Shirai, Ryota Nomura, Yukio Kato, Masaru Murakami, Chihiro Kondo, Soraaki Takahashi, Yoshie Yamasaki, Michiyo Nakano, Nobuaki Arai, Hidemi Yasuda, Kazuhiko Nakano, Fumitoshi Asai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Porphyromonas gulae, a suspected pathogen for periodontal disease in dogs, possesses approximately 41-kDa fimbriae (FimA) that are encoded by the fimA gene. In the present study, the association of fimA genotypes with mitral regurgitation (MR) was investigated. Twenty-five dogs diagnosed with MR (age range 6-13 years old, average 10.8 years) and 32 healthy dogs (8-15 years old, average 10.8 years) were selected at the participating clinics in a consecutive manner during the same time period. Oral swab specimens were collected from the dogs and bacterial DNA was extracted, then polymerase chain reaction analysis was performed using primers specific for each fimA genotype, with the dominant genotype determined. The rate for genotype C dominant specimens was 48.0% in the MR group, which was significantly higher than that in the control group (18.8%) (P <0.05). These results suggest that P gulae fimA genotype C is associated with MR.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalResearch in Veterinary Science
Volume102
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Porphyromonas gulae
Porphyromonas
Mitral Valve Insufficiency
mouth
Communication
Genotype
Dogs
dogs
genotype
Bacterial DNA
fimbriae
Periodontal Diseases
polymerase chain reaction
Polymerase Chain Reaction
Control Groups
pathogens
DNA
Genes

Keywords

  • Fimbriae
  • Mitral regurgitation
  • Periodontal disease
  • Porphyromonas gulae

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Short communication : Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation. / Shirai, Mitsuyuki; Nomura, Ryota; Kato, Yukio; Murakami, Masaru; Kondo, Chihiro; Takahashi, Soraaki; Yamasaki, Yoshie; Nakano, Michiyo; Arai, Nobuaki; Yasuda, Hidemi; Nakano, Kazuhiko; Asai, Fumitoshi.

In: Research in Veterinary Science, Vol. 102, 01.10.2015, p. 49-52.

Research output: Contribution to journalArticle

Shirai, M, Nomura, R, Kato, Y, Murakami, M, Kondo, C, Takahashi, S, Yamasaki, Y, Nakano, M, Arai, N, Yasuda, H, Nakano, K & Asai, F 2015, 'Short communication: Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation', Research in Veterinary Science, vol. 102, pp. 49-52. https://doi.org/10.1016/j.rvsc.2015.07.009
Shirai, Mitsuyuki ; Nomura, Ryota ; Kato, Yukio ; Murakami, Masaru ; Kondo, Chihiro ; Takahashi, Soraaki ; Yamasaki, Yoshie ; Nakano, Michiyo ; Arai, Nobuaki ; Yasuda, Hidemi ; Nakano, Kazuhiko ; Asai, Fumitoshi. / Short communication : Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation. In: Research in Veterinary Science. 2015 ; Vol. 102. pp. 49-52.
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