Shoot formation from root tip region: A developmental alteration by WUS in transgenic tobacco

Syeda Zinia Rashid, Naoki Yamaji, Masaharu Kyo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined the effect of ectopic expression of WUS on the morphology of tobacco seedlings and the segments in vitro. WUS was amplified from Arabidopsis cDNA and introduced into the tobacco genome under the transcriptional control of the β-estradiol-inducible expression system. When 1-week-old transgenic seedlings were cultured in the presence of β-estradiol, only the root tip region developed bulbous tissues followed by shoot formation and plant regeneration, suggesting its applicability for improving the strategy of micropropagation in recalcitrant species. Evident abnormality was not observed in the cotyledons, hypocotyl nor root except for the tip. However, ectopic WUS seemed to be functional in those parts through the observation of gene expression and the behavior of cultured segments. Small root segments with a root tip treated with β-estradiol also showed bulbing but no shoots unless exogenous cytokinin was supplied. These findings suggest the existence of unknown factors regulating ectopic WUS function in the seedling.

Original languageEnglish
Pages (from-to)1449-1455
Number of pages7
JournalPlant Cell Reports
Volume26
Issue number9
DOIs
Publication statusPublished - Sep 1 2007
Externally publishedYes

Keywords

  • Ectopic expression
  • Nicotiana tabacum
  • Root tip
  • Shoot formation
  • Transdifferentiation
  • WUSCHEL

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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