Abstract
Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice. Materials and methods: We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results: SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions: Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 428-434 |
| Number of pages | 7 |
| Journal | Oral Diseases |
| Volume | 14 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jul 2008 |
| Externally published | Yes |
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Keywords
- Bone
- Osteoblast
- Regeneration
- Stem cells from human exfoliated deciduous teeth (SHED)
ASJC Scopus subject areas
- Dentistry(all)
Cite this
SHED repair critical-size calvarial defects in mice. / Seo, B. M.; Sonoyama, W.; Yamaza, T.; Coppe, C.; Kikuiri, T.; Akiyama, Kentaro; Lee, J. S.; Shi, S.
In: Oral Diseases, Vol. 14, No. 5, 07.2008, p. 428-434.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SHED repair critical-size calvarial defects in mice
AU - Seo, B. M.
AU - Sonoyama, W.
AU - Yamaza, T.
AU - Coppe, C.
AU - Kikuiri, T.
AU - Akiyama, Kentaro
AU - Lee, J. S.
AU - Shi, S.
PY - 2008/7
Y1 - 2008/7
N2 - Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice. Materials and methods: We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results: SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions: Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.
AB - Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice. Materials and methods: We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results: SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions: Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.
KW - Bone
KW - Osteoblast
KW - Regeneration
KW - Stem cells from human exfoliated deciduous teeth (SHED)
UR - http://www.scopus.com/inward/record.url?scp=45249119051&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45249119051&partnerID=8YFLogxK
U2 - 10.1111/j.1601-0825.2007.01396.x
DO - 10.1111/j.1601-0825.2007.01396.x
M3 - Article
C2 - 18938268
AN - SCOPUS:45249119051
VL - 14
SP - 428
EP - 434
JO - Oral Diseases
JF - Oral Diseases
SN - 1354-523X
IS - 5
ER -