Abstract
Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice. Materials and methods: We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results: SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions: Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.
Original language | English |
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Pages (from-to) | 428-434 |
Number of pages | 7 |
Journal | Oral Diseases |
Volume | 14 |
Issue number | 5 |
DOIs | |
Publication status | Published - Jul 2008 |
Externally published | Yes |
Keywords
- Bone
- Osteoblast
- Regeneration
- Stem cells from human exfoliated deciduous teeth (SHED)
ASJC Scopus subject areas
- Otorhinolaryngology
- Dentistry(all)