SHED repair critical-size calvarial defects in mice

B. M. Seo, W. Sonoyama, T. Yamaza, C. Coppe, T. Kikuiri, K. Akiyama, J. S. Lee, S. Shi

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Objective: Stem cells from human exfoliated deciduous teeth (SHED) are a population of highly proliferative postnatal stem cells capable of differentiating into odontoblasts, adipocytes, neural cells, and osteo-inductive cells. To examine whether SHED-mediated bone regeneration can be utilized for therapeutic purposes, we used SHED to repair critical-size calvarial defects in immunocompromised mice. Materials and methods: We generated calvarial defects and transplanted SHED with hydroxyapatite/tricalcium phosphate as a carrier into the defect areas. Results: SHED were able to repair the defects with substantial bone formation. Interestingly, SHED-mediated osteogenesis failed to recruit hematopoietic marrow elements that are commonly seen in bone marrow mesenchymal stem cell-generated bone. Furthermore, SHED were found to co-express mesenchymal stem cell marker, CC9/MUC18/CD146, with an array of growth factor receptors such as transforming growth factor β receptor I and II, fibroblast growth factor receptor I and III, and vascular endothelial growth factor receptor I, implying their comprehensive differentiation potential. Conclusions: Our data indicate that SHED, derived from neural crest cells, may select unique mechanisms to exert osteogenesis. SHED might be a suitable resource for orofacial bone regeneration.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalOral Diseases
Volume14
Issue number5
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Bone
  • Osteoblast
  • Regeneration
  • Stem cells from human exfoliated deciduous teeth (SHED)

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)

Fingerprint Dive into the research topics of 'SHED repair critical-size calvarial defects in mice'. Together they form a unique fingerprint.

  • Cite this

    Seo, B. M., Sonoyama, W., Yamaza, T., Coppe, C., Kikuiri, T., Akiyama, K., Lee, J. S., & Shi, S. (2008). SHED repair critical-size calvarial defects in mice. Oral Diseases, 14(5), 428-434. https://doi.org/10.1111/j.1601-0825.2007.01396.x