Shape of α-crystallin analyzed by small-angle neutron scattering

Masaaki Sugiyama, Noriko Fujii, Yukio Morimoto, Toshiya Otomo, Shinichi Takata, Masakatsu Misawa, Masahiko Annaka, Keiji Itoh, Kazuhiro Mori, Takashi Sato, Sakie Kurabayashi, Toshiharu Fukunaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The size and shape of aggregates of human recombinant αA-crystallin and αB-crystallin are investigated with small-angle neutron scattering and dynamic light scattering. At a bioactive temperature (310 K), both polypeptides form aggregates with almost the same size and shape. The αB-crystallin maintains an almost identical size and shape at 310 and 288 K, whereas the aggregate of αA-crystallin shows deformation at 288 K. This result suggests that at the lower temperature there is a difference in structural stability between the two aggregates of the polypeptides.

Original languageEnglish
JournalJournal of Applied Crystallography
Volume40
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Apr 2007
Externally publishedYes

Fingerprint

Small Angle Scattering
Crystallins
Neutrons
Neutron scattering
neutron scattering
polypeptides
Peptides
Temperature
structural stability
Dynamic light scattering
activity (biology)
light scattering
temperature

Keywords

  • αA-crystallin
  • αB-crystallin
  • Dynamic light scattering
  • Small-angle neutron scattering

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Structural Biology

Cite this

Sugiyama, M., Fujii, N., Morimoto, Y., Otomo, T., Takata, S., Misawa, M., ... Fukunaga, T. (2007). Shape of α-crystallin analyzed by small-angle neutron scattering. Journal of Applied Crystallography, 40(SUPPL. 1). https://doi.org/10.1107/S0021889807006838

Shape of α-crystallin analyzed by small-angle neutron scattering. / Sugiyama, Masaaki; Fujii, Noriko; Morimoto, Yukio; Otomo, Toshiya; Takata, Shinichi; Misawa, Masakatsu; Annaka, Masahiko; Itoh, Keiji; Mori, Kazuhiro; Sato, Takashi; Kurabayashi, Sakie; Fukunaga, Toshiharu.

In: Journal of Applied Crystallography, Vol. 40, No. SUPPL. 1, 04.2007.

Research output: Contribution to journalArticle

Sugiyama, M, Fujii, N, Morimoto, Y, Otomo, T, Takata, S, Misawa, M, Annaka, M, Itoh, K, Mori, K, Sato, T, Kurabayashi, S & Fukunaga, T 2007, 'Shape of α-crystallin analyzed by small-angle neutron scattering', Journal of Applied Crystallography, vol. 40, no. SUPPL. 1. https://doi.org/10.1107/S0021889807006838
Sugiyama M, Fujii N, Morimoto Y, Otomo T, Takata S, Misawa M et al. Shape of α-crystallin analyzed by small-angle neutron scattering. Journal of Applied Crystallography. 2007 Apr;40(SUPPL. 1). https://doi.org/10.1107/S0021889807006838
Sugiyama, Masaaki ; Fujii, Noriko ; Morimoto, Yukio ; Otomo, Toshiya ; Takata, Shinichi ; Misawa, Masakatsu ; Annaka, Masahiko ; Itoh, Keiji ; Mori, Kazuhiro ; Sato, Takashi ; Kurabayashi, Sakie ; Fukunaga, Toshiharu. / Shape of α-crystallin analyzed by small-angle neutron scattering. In: Journal of Applied Crystallography. 2007 ; Vol. 40, No. SUPPL. 1.
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