SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Naoyoshi Kumakura, Atsushi Takeda, Yoichiro Fujioka, Hiroyasu Motose, Ryo Takano, Yuichiro Watanabe

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. Structured summary: MINT-7014710: SGS3 (uniprotkb:Q9LDX1) and RDR6 (uniprotkb:Q9SG02) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809). MINT-7014697: RDR6 (uniprotkb:Q9SG02) and SGS3 (uniprotkb:Q9LDX1) colocalize (MI:0403) by fluorescence microscopy (MI:0416).

Original languageEnglish
Pages (from-to)1261-1266
Number of pages6
JournalFEBS Letters
Volume583
Issue number8
DOIs
Publication statusPublished - Apr 17 2009
Externally publishedYes

Fingerprint

Suppressor Genes
RNA Replicase
Gene Silencing
Genes
Small Interfering RNA
Cytoplasmic Granules
Fluorescence microscopy
Fluorescence Microscopy
Arabidopsis
Fluorescence
Messenger RNA

Keywords

  • Arabidopsis
  • Dicer-like 4
  • Processing body
  • RNA silencing
  • RNA-dependent RNA polymerase 6
  • Suppressor of gene silencing 3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Kumakura, N., Takeda, A., Fujioka, Y., Motose, H., Takano, R., & Watanabe, Y. (2009). SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies. FEBS Letters, 583(8), 1261-1266. https://doi.org/10.1016/j.febslet.2009.03.055

SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies. / Kumakura, Naoyoshi; Takeda, Atsushi; Fujioka, Yoichiro; Motose, Hiroyasu; Takano, Ryo; Watanabe, Yuichiro.

In: FEBS Letters, Vol. 583, No. 8, 17.04.2009, p. 1261-1266.

Research output: Contribution to journalArticle

Kumakura, N, Takeda, A, Fujioka, Y, Motose, H, Takano, R & Watanabe, Y 2009, 'SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies', FEBS Letters, vol. 583, no. 8, pp. 1261-1266. https://doi.org/10.1016/j.febslet.2009.03.055
Kumakura, Naoyoshi ; Takeda, Atsushi ; Fujioka, Yoichiro ; Motose, Hiroyasu ; Takano, Ryo ; Watanabe, Yuichiro. / SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies. In: FEBS Letters. 2009 ; Vol. 583, No. 8. pp. 1261-1266.
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