Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ

Melissa A. Larson, Koji Kimura, H. Michael Kubisch, R. Michael Roberts

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

IFN-τ is a secretory product of trophectoderm of cattle, sheep, and their relatives and is expressed for a few days in early pregnancy after the blastocyst first forms. It serves to alert the mother that she is pregnant. A delayed or less than robust IFN-τ signal is a likely cause of embryonic loss. Here we have determined whether blastocyst production of IFN-τ, which is encoded by a cluster of genes on chromosome 9, differs between the sexes in cattle, as assessed by culture of in vitro-derived embryos on two different media, one complex (tissue culture medium 199 supplemented with serum) with coculture support, the other relatively simple (synthetic oviductal fluid plus albumin). With both media, female blastocysts produced approximately double the amount of IFN-τ as males, regardless of such variables as oocyte batch, blastocyst quality, hatching, and length of time in culture. However, in either tissue culture medium 199, which contains 5.5 mM D-glucose, or in synthetic oviductal fluid, in the presence but not in the absence of added glucose, significantly fewer female than male embryos were able to progress from the morula/early blastocyst stage to more advanced stages of development. It is possible that the differences between male and female embryos both in their production of IFN-τ and in their ability to progress in development in glucose-rich media are manifestations of phenomena that occur in vivo and provide plasticity in embryo selection during early pregnancy.

Original languageEnglish
Pages (from-to)9677-9682
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number17
DOIs
Publication statusPublished - Aug 14 2001
Externally publishedYes

Fingerprint

Blastocyst
Sex Characteristics
Embryonic Structures
Glucose
Culture Media
Morula
Pregnancy
Chromosomes, Human, Pair 9
Multigene Family
Coculture Techniques
Oocytes
Albumins
Sheep
Serum

Keywords

  • Embryo culture
  • In vitro maturation-in vitro fertilization
  • Sexual dimorphism

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{b7c946b52f2547fcb756f4abf3640c2f,
title = "Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ",
abstract = "IFN-τ is a secretory product of trophectoderm of cattle, sheep, and their relatives and is expressed for a few days in early pregnancy after the blastocyst first forms. It serves to alert the mother that she is pregnant. A delayed or less than robust IFN-τ signal is a likely cause of embryonic loss. Here we have determined whether blastocyst production of IFN-τ, which is encoded by a cluster of genes on chromosome 9, differs between the sexes in cattle, as assessed by culture of in vitro-derived embryos on two different media, one complex (tissue culture medium 199 supplemented with serum) with coculture support, the other relatively simple (synthetic oviductal fluid plus albumin). With both media, female blastocysts produced approximately double the amount of IFN-τ as males, regardless of such variables as oocyte batch, blastocyst quality, hatching, and length of time in culture. However, in either tissue culture medium 199, which contains 5.5 mM D-glucose, or in synthetic oviductal fluid, in the presence but not in the absence of added glucose, significantly fewer female than male embryos were able to progress from the morula/early blastocyst stage to more advanced stages of development. It is possible that the differences between male and female embryos both in their production of IFN-τ and in their ability to progress in development in glucose-rich media are manifestations of phenomena that occur in vivo and provide plasticity in embryo selection during early pregnancy.",
keywords = "Embryo culture, In vitro maturation-in vitro fertilization, Sexual dimorphism",
author = "Larson, {Melissa A.} and Koji Kimura and Kubisch, {H. Michael} and Roberts, {R. Michael}",
year = "2001",
month = "8",
day = "14",
doi = "10.1073/pnas.171305398",
language = "English",
volume = "98",
pages = "9677--9682",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "17",

}

TY - JOUR

T1 - Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ

AU - Larson, Melissa A.

AU - Kimura, Koji

AU - Kubisch, H. Michael

AU - Roberts, R. Michael

PY - 2001/8/14

Y1 - 2001/8/14

N2 - IFN-τ is a secretory product of trophectoderm of cattle, sheep, and their relatives and is expressed for a few days in early pregnancy after the blastocyst first forms. It serves to alert the mother that she is pregnant. A delayed or less than robust IFN-τ signal is a likely cause of embryonic loss. Here we have determined whether blastocyst production of IFN-τ, which is encoded by a cluster of genes on chromosome 9, differs between the sexes in cattle, as assessed by culture of in vitro-derived embryos on two different media, one complex (tissue culture medium 199 supplemented with serum) with coculture support, the other relatively simple (synthetic oviductal fluid plus albumin). With both media, female blastocysts produced approximately double the amount of IFN-τ as males, regardless of such variables as oocyte batch, blastocyst quality, hatching, and length of time in culture. However, in either tissue culture medium 199, which contains 5.5 mM D-glucose, or in synthetic oviductal fluid, in the presence but not in the absence of added glucose, significantly fewer female than male embryos were able to progress from the morula/early blastocyst stage to more advanced stages of development. It is possible that the differences between male and female embryos both in their production of IFN-τ and in their ability to progress in development in glucose-rich media are manifestations of phenomena that occur in vivo and provide plasticity in embryo selection during early pregnancy.

AB - IFN-τ is a secretory product of trophectoderm of cattle, sheep, and their relatives and is expressed for a few days in early pregnancy after the blastocyst first forms. It serves to alert the mother that she is pregnant. A delayed or less than robust IFN-τ signal is a likely cause of embryonic loss. Here we have determined whether blastocyst production of IFN-τ, which is encoded by a cluster of genes on chromosome 9, differs between the sexes in cattle, as assessed by culture of in vitro-derived embryos on two different media, one complex (tissue culture medium 199 supplemented with serum) with coculture support, the other relatively simple (synthetic oviductal fluid plus albumin). With both media, female blastocysts produced approximately double the amount of IFN-τ as males, regardless of such variables as oocyte batch, blastocyst quality, hatching, and length of time in culture. However, in either tissue culture medium 199, which contains 5.5 mM D-glucose, or in synthetic oviductal fluid, in the presence but not in the absence of added glucose, significantly fewer female than male embryos were able to progress from the morula/early blastocyst stage to more advanced stages of development. It is possible that the differences between male and female embryos both in their production of IFN-τ and in their ability to progress in development in glucose-rich media are manifestations of phenomena that occur in vivo and provide plasticity in embryo selection during early pregnancy.

KW - Embryo culture

KW - In vitro maturation-in vitro fertilization

KW - Sexual dimorphism

UR - http://www.scopus.com/inward/record.url?scp=0035859882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035859882&partnerID=8YFLogxK

U2 - 10.1073/pnas.171305398

DO - 10.1073/pnas.171305398

M3 - Article

C2 - 11481449

AN - SCOPUS:0035859882

VL - 98

SP - 9677

EP - 9682

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 17

ER -