TY - JOUR
T1 - Sex- or age-related differences were not detected in the activity of dihydropyrimidine dehydrogenase from rat liver
AU - Tateishi, Tomonori
AU - Watanabe, Minoru
AU - Nakura, Hironori
AU - Tanaka, Masami
AU - Kumai, Toshio
AU - Kobayashi, Shinichi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997/2
Y1 - 1997/2
N2 - We examined the influence of ageing and gender on the activity of dihydropyrimidine dehydrogenase (DPD) in male and female Sprague-Dawley rats at 3, 8, 40 and older than 60 weeks of age. The body and liver weights of male rats at 8 weeks and older of age were heavier than those of female rats at the same age and the ratio of liver to body weight was significantly reduced with ageing. The amount of produced 5FU metabolites, dihydrofluorouracil and fluoroureidopropionic acid, remained unchanged in the reaction mixture with the cytosol from any group of the rats. The formation rates of 5FU metabolite were 1.03 ± 0.08 / 1.12 ± 0.06, 0.98 ± 0.11 / 1.16 ± 0.10, 1.13 ± 0.09 / 1.13 ± 0.11 and 1.17 ± 0.16 / 1.24 ± 0.08 nmol min-1 mg protein-1 (mean ± SD, male/female) in rats at 3, 8, 40 and older than 60 weeks of age, respectively. The results suggest that the DPD activity in the rat liver may not be affected by ageing or gender.
AB - We examined the influence of ageing and gender on the activity of dihydropyrimidine dehydrogenase (DPD) in male and female Sprague-Dawley rats at 3, 8, 40 and older than 60 weeks of age. The body and liver weights of male rats at 8 weeks and older of age were heavier than those of female rats at the same age and the ratio of liver to body weight was significantly reduced with ageing. The amount of produced 5FU metabolites, dihydrofluorouracil and fluoroureidopropionic acid, remained unchanged in the reaction mixture with the cytosol from any group of the rats. The formation rates of 5FU metabolite were 1.03 ± 0.08 / 1.12 ± 0.06, 0.98 ± 0.11 / 1.16 ± 0.10, 1.13 ± 0.09 / 1.13 ± 0.11 and 1.17 ± 0.16 / 1.24 ± 0.08 nmol min-1 mg protein-1 (mean ± SD, male/female) in rats at 3, 8, 40 and older than 60 weeks of age, respectively. The results suggest that the DPD activity in the rat liver may not be affected by ageing or gender.
KW - 5-Fluorouracil
KW - Ageing
KW - Dihydropyrimidine dehydrogenase
KW - Gender difference
KW - Sprague-Dawley rats
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U2 - 10.1006/phrs.1996.0109
DO - 10.1006/phrs.1996.0109
M3 - Article
C2 - 9175577
AN - SCOPUS:0030949968
VL - 35
SP - 103
EP - 106
JO - Pharmacological Research
JF - Pharmacological Research
SN - 1043-6618
IS - 2
ER -