Sex differentiation and mRNA expression of P450c17, P450arom and AMH in gonads of the chicken

Hiromi Nishikimi, Norio Kansaku, Noboru Saito, Makoto Usami, Yasuo Ohno, Kiyoshi Shimada

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017α-hydroxylase (P450c17), P450 aromatase (P450arom) and anti-Mullerian hormone (AMH)in the chicken gonads. The mRNA levels in the gonads at days 4-8 of incubation were assessed by in situ hybridization analysis using digoxigenin labeling method. The in situ hybridization data were analyzed by relative expression of specific hybridizable signals of each mRNA corrected by the non-specific background by employing an image analyzer. P450c17 mRNA expression increased rapidly at day 6 of incubation in the male but decreased thereafter. In contrast to the transient expression in the male, the expression was gradually increased in the female. P450arom mRNA was not expressed in the male but was detectable in the female as early as day 6 and increased subsequently with days of incubation. AMH mRNA was expressed as early as day 5 of incubation followed by a sharp increase on day 6, which was maintained in the male thereafter. In contrast, the female showed very little expression. The injection of Fadrozole caused no effect on P450c 17 mRNA expression, while it suppressed P450arom mRNA expression but increased AMH mRNA expression in the female. In contrast, the injection of estradiol induced P450arom mRNA expression significantly but suppressed AMH mRNA expression in the male. These results indicate that expression of P450arom and AMH is sexually dim orphic and is reciprocally regulated during early ontogenic life in chicken gonads.

Original languageEnglish
Pages (from-to)20-30
Number of pages11
JournalMolecular Reproduction and Development
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 1 2000
Externally publishedYes

Keywords

  • AMH
  • Chicken embryo
  • Gonads
  • MRNA
  • P450arom
  • Sex differentiation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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