Sex- and tissue-specific expression of P450 aromatase (cyp19a1a) in the yellowtail clownfish, Amphiprion clarkii

Yasuhisa Kobayashi, Ryo Horiguchi, Saori Miura, Masaru Nakamura

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26 Citations (Scopus)


To investigate the role of estrogen in the gonad of yellowtail clownfish Amphiprion clarkii, we isolated cDNA encoding cytochrome P450 aromatase (Cyp19a1a) from the adult ovary. The full-length cDNA of clownfish cyp19a1a is 1928-bp long and encodes 520 amino acids. Real-time quantitative RT-PCR analysis showed that cyp19a1a was expressed mainly in the ovary of female-phase fish. In situ hybridization and immunohistochemical observations showed that positive signals were restricted to the ovarian follicle of the female-phase fish. In contrast, Cyp19a1a signal was not detected in the ambisexual gonad of the male-phase fish. These findings suggest that Cyp19a1a is involved in oogenesis in the female-phase fish, but not in the ambisexual gonad of male-phase fish.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Issue number2
Publication statusPublished - Feb 2010
Externally publishedYes



  • Aromatase
  • Clownfish
  • cyp19a1a
  • Sex change
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

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