Sex- and tissue-specific expression of P450 aromatase (cyp19a1a) in the yellowtail clownfish, Amphiprion clarkii

Yasuhisa Kobayashi, Ryo Horiguchi, Saori Miura, Masaru Nakamura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To investigate the role of estrogen in the gonad of yellowtail clownfish Amphiprion clarkii, we isolated cDNA encoding cytochrome P450 aromatase (Cyp19a1a) from the adult ovary. The full-length cDNA of clownfish cyp19a1a is 1928-bp long and encodes 520 amino acids. Real-time quantitative RT-PCR analysis showed that cyp19a1a was expressed mainly in the ovary of female-phase fish. In situ hybridization and immunohistochemical observations showed that positive signals were restricted to the ovarian follicle of the female-phase fish. In contrast, Cyp19a1a signal was not detected in the ambisexual gonad of the male-phase fish. These findings suggest that Cyp19a1a is involved in oogenesis in the female-phase fish, but not in the ambisexual gonad of male-phase fish.

Original languageEnglish
Pages (from-to)237-244
Number of pages8
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume155
Issue number2
DOIs
Publication statusPublished - Feb 2010
Externally publishedYes

Fingerprint

Aromatase
Fish
Fishes
Tissue
Gonads
Ovary
Complementary DNA
Oogenesis
Ovarian Follicle
Cytochrome P-450 Enzyme System
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Estrogens
Amino Acids

Keywords

  • Aromatase
  • Clownfish
  • cyp19a1a
  • Sex change
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

Sex- and tissue-specific expression of P450 aromatase (cyp19a1a) in the yellowtail clownfish, Amphiprion clarkii. / Kobayashi, Yasuhisa; Horiguchi, Ryo; Miura, Saori; Nakamura, Masaru.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 155, No. 2, 02.2010, p. 237-244.

Research output: Contribution to journalArticle

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