Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer

Atsuki Ikeda, Shin Nishiumi, Masakazu Shinohara, Tomoo Yoshie, Naoya Hatano, Tatsuya Okuno, Takeshi Bamba, Eiichiro Fukusaki, Tadaomi Takenawa, Takeshi Azuma, Masaru Yoshida

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Conventional tumor markers are unsuitable for detecting carcinoma at an early stage and lack clinical efficacy and utility. In this study, we attempted to investigate the differences in serum metabolite profiles of gastrointestinal cancers and healthy volunteers using a metabolomic approach and searched for sensitive and specific metabolomic biomarker candidates. Human serum samples were obtained esophageal (n=15), gastric (n=11), and colorectal (n=12) cancer patients and healthy volunteers (n=12). A model for evaluating metabolomic biomarker candidates was constructed using multiple classification analysis, and the results were assessed with receiver operating characteristic curves. Among the 58 metabolites, the levels of nine, five and 12 metabolites were significantly changed in the esophageal, gastric and colorectal cancer patients, respectively, compared with the healthy volunteers. Multiple classification analysis revealed that the variations in the levels of malonic acid and l-serine largely contributed to the separation of esophageal cancer; gastric cancer was characterized by changes in the levels of 3-hydroxypropionic acid and pyruvic acid; and l-alanine, glucuronoic lactone and l-glutamine contributed to the separation of colorectal cancer. Our approach revealed that some metabolites are more sensitive for detecting gastrointestinal cancer than conventional biomarkers. Our study supports the potential of metabolomics as an early diagnostic tool for cancer.

Original languageEnglish
Pages (from-to)548-558
Number of pages11
JournalBiomedical Chromatography
Volume26
Issue number5
DOIs
Publication statusPublished - May 1 2012
Externally publishedYes

Fingerprint

Gastrointestinal Neoplasms
Metabolomics
Metabolites
Healthy Volunteers
Biomarkers
Esophageal Neoplasms
Tumor Biomarkers
Serum
Stomach Neoplasms
Colorectal Neoplasms
Lactones
Glutamine
Pyruvic Acid
ROC Curve
Alanine
Serine
Neoplasms
Stomach
Carcinoma

Keywords

  • Biomarker
  • GC/MS
  • Multiple classification analysis
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

Ikeda, A., Nishiumi, S., Shinohara, M., Yoshie, T., Hatano, N., Okuno, T., ... Yoshida, M. (2012). Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer. Biomedical Chromatography, 26(5), 548-558. https://doi.org/10.1002/bmc.1671

Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer. / Ikeda, Atsuki; Nishiumi, Shin; Shinohara, Masakazu; Yoshie, Tomoo; Hatano, Naoya; Okuno, Tatsuya; Bamba, Takeshi; Fukusaki, Eiichiro; Takenawa, Tadaomi; Azuma, Takeshi; Yoshida, Masaru.

In: Biomedical Chromatography, Vol. 26, No. 5, 01.05.2012, p. 548-558.

Research output: Contribution to journalArticle

Ikeda, A, Nishiumi, S, Shinohara, M, Yoshie, T, Hatano, N, Okuno, T, Bamba, T, Fukusaki, E, Takenawa, T, Azuma, T & Yoshida, M 2012, 'Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer', Biomedical Chromatography, vol. 26, no. 5, pp. 548-558. https://doi.org/10.1002/bmc.1671
Ikeda, Atsuki ; Nishiumi, Shin ; Shinohara, Masakazu ; Yoshie, Tomoo ; Hatano, Naoya ; Okuno, Tatsuya ; Bamba, Takeshi ; Fukusaki, Eiichiro ; Takenawa, Tadaomi ; Azuma, Takeshi ; Yoshida, Masaru. / Serum metabolomics as a novel diagnostic approach for gastrointestinal cancer. In: Biomedical Chromatography. 2012 ; Vol. 26, No. 5. pp. 548-558.
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