Serum hydroxyl radical scavenging capacity as quantified with iron-free hydroxyl radical source

Nobuyuki Endo, Shigeru Oowada, Yoshimi Sueishi, Masashi Shimmei, Keisuke Makino, Hirotada Fujii, Yashige Kotake

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have developed a simple ESR spin trapping based method for hydroxyl (OH) radical scavenging-capacity determination, using iron-free OH radical source. Instead of the widely used Fenton reaction, a short (typically 5 seconds) in situ UV-photolysis of a dilute hydrogen peroxide aqueous solution was employed to generate reproducible amounts of OH radicals. ESR spin trapping was applied to quantify OH radicals; the decrease in the OH radical level due to the specimen's scavenging activity was converted into the OH radical scavenging capacity (rate). The validity of the method was confirmed in pure antioxidants, and the agreement with the previous data was satisfactory. In the second half of this work, the new method was applied to the sera of chronic renal failure (CRF) patients. We show for the first time that after hemodialysis, OH radical scavenging capacity of the CRF serum was restored to the level of healthy control. This method is simple and rapid, and the low concentration hydrogen peroxide is the only chemical added to the system, that could eliminate the complexity of iron-involved Fenton reactions or the use of the pulse-radiolysis system.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalJournal of Clinical Biochemistry and Nutrition
Volume45
Issue number2
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Scavenging
Free radicals
Hydroxyl Radical
Free Radicals
Iron
Spin Trapping
Serum
Hydrogen Peroxide
Chronic Kidney Failure
Paramagnetic resonance
Pulse Radiolysis
Radiolysis
Photolysis
Renal Dialysis
Antioxidants

Keywords

  • Antioxidant capacity
  • ESR spin trapping
  • Hydrogen peroxide
  • Hydroxyl radical
  • Photochemistry

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Serum hydroxyl radical scavenging capacity as quantified with iron-free hydroxyl radical source. / Endo, Nobuyuki; Oowada, Shigeru; Sueishi, Yoshimi; Shimmei, Masashi; Makino, Keisuke; Fujii, Hirotada; Kotake, Yashige.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 45, No. 2, 09.2009, p. 193-201.

Research output: Contribution to journalArticle

Endo, Nobuyuki ; Oowada, Shigeru ; Sueishi, Yoshimi ; Shimmei, Masashi ; Makino, Keisuke ; Fujii, Hirotada ; Kotake, Yashige. / Serum hydroxyl radical scavenging capacity as quantified with iron-free hydroxyl radical source. In: Journal of Clinical Biochemistry and Nutrition. 2009 ; Vol. 45, No. 2. pp. 193-201.
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