Serum Glycan Markers for Evaluation of Disease Activity and Prediction of Clinical Course in Patients with Ulcerative Colitis

Koji Miyahara, Kazuhiro Nouso, Shunsuke Saito, Sakiko Hiraoka, Keita Harada, Sakuma Takahashi, Yuki Morimoto, Sayo Kobayashi, Fusao Ikeda, Yasuhiro Miyake, Hidenori Shiraha, Akinobu Takaki, Hiroyuki Okada, Maho Amano, Kazuko Hirose, Shin Ichiro Nishimura, Kazuhide Yamamoto

Research output: Contribution to journalArticle

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Abstract

Background:The aims of this study were to determine the change of whole-serum N-glycan profile in ulcerative colitis (UC) patients and to investigate its clinical utility.Methods:We collected serum from 75 UC patients at the time of admission and the same number of age/sex-matched healthy volunteers. Serum glycan profile was measured by comprehensive quantitative high-throughput glycome analysis and was compared with disease activity and prognosis.Results:Out of 61 glycans detected, 24 were differentially expressed in UC patients. Pathway analysis demonstrated that highly sialylated multi-branched glycans and agalactosyl bi-antennary glycans were elevated in UC patients; in addition, the glycan ratio m/z 2378/1914, which also increased in UC, showed the highest Area under Receiver Operating Characteristic curve (0.923) for the diagnosis of UC. Highly sialylated multi-branched glycans and the glycan ratio m/z 2378/1914 were higher in the patients with total colitis, Clinical Activity Index >10, Mayo endoscopic score 3, or a steroid-refractory status. In particular, the glycan ratio m/z 2378/1914 (above median) was an independent prognostic factor for the need for an operation (hazard ratio, 2.67; 95% confidence interval, 1.04-7.84).Conclusions:Whole-serum glycan profiles revealed that the glycan ratio m/z 2378/1914 and highly sialylated multi-branched glycans increase in UC patients, and are correlated with disease activity. The glycan ratio m/z 2378/1914 was an independent predictive factor of the prognosis of UC.

Original languageEnglish
Article numbere74861
JournalPLoS One
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 7 2013

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colitis
Ulcerative Colitis
disease course
Polysaccharides
polysaccharides
Biomarkers
prediction
Serum
prognosis
Colitis
ROC Curve
Refractory materials
volunteers
steroids
confidence interval
Hazards
Healthy Volunteers
Steroids
Throughput

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Serum Glycan Markers for Evaluation of Disease Activity and Prediction of Clinical Course in Patients with Ulcerative Colitis. / Miyahara, Koji; Nouso, Kazuhiro; Saito, Shunsuke; Hiraoka, Sakiko; Harada, Keita; Takahashi, Sakuma; Morimoto, Yuki; Kobayashi, Sayo; Ikeda, Fusao; Miyake, Yasuhiro; Shiraha, Hidenori; Takaki, Akinobu; Okada, Hiroyuki; Amano, Maho; Hirose, Kazuko; Nishimura, Shin Ichiro; Yamamoto, Kazuhide.

In: PLoS One, Vol. 8, No. 10, e74861, 07.10.2013.

Research output: Contribution to journalArticle

Miyahara, K, Nouso, K, Saito, S, Hiraoka, S, Harada, K, Takahashi, S, Morimoto, Y, Kobayashi, S, Ikeda, F, Miyake, Y, Shiraha, H, Takaki, A, Okada, H, Amano, M, Hirose, K, Nishimura, SI & Yamamoto, K 2013, 'Serum Glycan Markers for Evaluation of Disease Activity and Prediction of Clinical Course in Patients with Ulcerative Colitis', PLoS One, vol. 8, no. 10, e74861. https://doi.org/10.1371/journal.pone.0074861
Miyahara, Koji ; Nouso, Kazuhiro ; Saito, Shunsuke ; Hiraoka, Sakiko ; Harada, Keita ; Takahashi, Sakuma ; Morimoto, Yuki ; Kobayashi, Sayo ; Ikeda, Fusao ; Miyake, Yasuhiro ; Shiraha, Hidenori ; Takaki, Akinobu ; Okada, Hiroyuki ; Amano, Maho ; Hirose, Kazuko ; Nishimura, Shin Ichiro ; Yamamoto, Kazuhide. / Serum Glycan Markers for Evaluation of Disease Activity and Prediction of Clinical Course in Patients with Ulcerative Colitis. In: PLoS One. 2013 ; Vol. 8, No. 10.
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abstract = "Background:The aims of this study were to determine the change of whole-serum N-glycan profile in ulcerative colitis (UC) patients and to investigate its clinical utility.Methods:We collected serum from 75 UC patients at the time of admission and the same number of age/sex-matched healthy volunteers. Serum glycan profile was measured by comprehensive quantitative high-throughput glycome analysis and was compared with disease activity and prognosis.Results:Out of 61 glycans detected, 24 were differentially expressed in UC patients. Pathway analysis demonstrated that highly sialylated multi-branched glycans and agalactosyl bi-antennary glycans were elevated in UC patients; in addition, the glycan ratio m/z 2378/1914, which also increased in UC, showed the highest Area under Receiver Operating Characteristic curve (0.923) for the diagnosis of UC. Highly sialylated multi-branched glycans and the glycan ratio m/z 2378/1914 were higher in the patients with total colitis, Clinical Activity Index >10, Mayo endoscopic score 3, or a steroid-refractory status. In particular, the glycan ratio m/z 2378/1914 (above median) was an independent prognostic factor for the need for an operation (hazard ratio, 2.67; 95{\%} confidence interval, 1.04-7.84).Conclusions:Whole-serum glycan profiles revealed that the glycan ratio m/z 2378/1914 and highly sialylated multi-branched glycans increase in UC patients, and are correlated with disease activity. The glycan ratio m/z 2378/1914 was an independent predictive factor of the prognosis of UC.",
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AU - Miyahara, Koji

AU - Nouso, Kazuhiro

AU - Saito, Shunsuke

AU - Hiraoka, Sakiko

AU - Harada, Keita

AU - Takahashi, Sakuma

AU - Morimoto, Yuki

AU - Kobayashi, Sayo

AU - Ikeda, Fusao

AU - Miyake, Yasuhiro

AU - Shiraha, Hidenori

AU - Takaki, Akinobu

AU - Okada, Hiroyuki

AU - Amano, Maho

AU - Hirose, Kazuko

AU - Nishimura, Shin Ichiro

AU - Yamamoto, Kazuhide

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Y1 - 2013/10/7

N2 - Background:The aims of this study were to determine the change of whole-serum N-glycan profile in ulcerative colitis (UC) patients and to investigate its clinical utility.Methods:We collected serum from 75 UC patients at the time of admission and the same number of age/sex-matched healthy volunteers. Serum glycan profile was measured by comprehensive quantitative high-throughput glycome analysis and was compared with disease activity and prognosis.Results:Out of 61 glycans detected, 24 were differentially expressed in UC patients. Pathway analysis demonstrated that highly sialylated multi-branched glycans and agalactosyl bi-antennary glycans were elevated in UC patients; in addition, the glycan ratio m/z 2378/1914, which also increased in UC, showed the highest Area under Receiver Operating Characteristic curve (0.923) for the diagnosis of UC. Highly sialylated multi-branched glycans and the glycan ratio m/z 2378/1914 were higher in the patients with total colitis, Clinical Activity Index >10, Mayo endoscopic score 3, or a steroid-refractory status. In particular, the glycan ratio m/z 2378/1914 (above median) was an independent prognostic factor for the need for an operation (hazard ratio, 2.67; 95% confidence interval, 1.04-7.84).Conclusions:Whole-serum glycan profiles revealed that the glycan ratio m/z 2378/1914 and highly sialylated multi-branched glycans increase in UC patients, and are correlated with disease activity. The glycan ratio m/z 2378/1914 was an independent predictive factor of the prognosis of UC.

AB - Background:The aims of this study were to determine the change of whole-serum N-glycan profile in ulcerative colitis (UC) patients and to investigate its clinical utility.Methods:We collected serum from 75 UC patients at the time of admission and the same number of age/sex-matched healthy volunteers. Serum glycan profile was measured by comprehensive quantitative high-throughput glycome analysis and was compared with disease activity and prognosis.Results:Out of 61 glycans detected, 24 were differentially expressed in UC patients. Pathway analysis demonstrated that highly sialylated multi-branched glycans and agalactosyl bi-antennary glycans were elevated in UC patients; in addition, the glycan ratio m/z 2378/1914, which also increased in UC, showed the highest Area under Receiver Operating Characteristic curve (0.923) for the diagnosis of UC. Highly sialylated multi-branched glycans and the glycan ratio m/z 2378/1914 were higher in the patients with total colitis, Clinical Activity Index >10, Mayo endoscopic score 3, or a steroid-refractory status. In particular, the glycan ratio m/z 2378/1914 (above median) was an independent prognostic factor for the need for an operation (hazard ratio, 2.67; 95% confidence interval, 1.04-7.84).Conclusions:Whole-serum glycan profiles revealed that the glycan ratio m/z 2378/1914 and highly sialylated multi-branched glycans increase in UC patients, and are correlated with disease activity. The glycan ratio m/z 2378/1914 was an independent predictive factor of the prognosis of UC.

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