Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus

Kazuyuki Sogawa, Kenta Noda, Hiroshi Umemura, Masanori Seimiya, Takahisa Kuga, Takeshi Tomonaga, Motoi Nishimura, Fumihiko Kanai, Fumio Imazeki, Hirotaka Takizawa, Masato Yoneda, Atsushi Nakajima, Mikihiro Tsutsumi, Osamu Yokosuka, Fumio Nomura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: Clinical application of biomarker candidates discovered by proteomic analysis is challenging. The purpose of this study was to standardize preanalytical conditions for measurement of serum levels of fibrinogen alpha C-chain 5.9 kDa fragment (FIC 5.9) and to test the diagnostic value of this peptide for detection of early hepatic fibrosis in patients with hepatitis C virus (HCV)-related chronic hepatitis. Experimental design: Serum FIC 5.9 levels were measured by a sandwich ELISA. Effects on the serum FIC 5.9 level of temperature, the time between venipuncture and serum separation, and the types of collection tubes used were examined. The diagnostic value of serum FIC 5.9 as an early indicator of hepatic fibrosis due to HCV was then assessed. Results: FIC 5.9 was produced in a time- and temperature-dependent manner after venipuncture. Abnormal FIC 5.9 values were found in 89.5% of FI stage patients. Receiver operating characteristic analyses confirmed the superiority of FIC 5.9 over other conventional markers for early detection of fibrosis. Conclusions and clinical relevance: The serum FIC 5.9 level may be an early indicator of hepatic fibrosis in HCV-related chronic liver diseases. This study provides an example of a pipeline from biomarker discovery by proteome analysis to assay optimization and preliminary clinical validation.

Original languageEnglish
Pages (from-to)424-431
Number of pages8
JournalProteomics - Clinical Applications
Volume7
Issue number5-6
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Chronic hepatitis due to hepatitis C virus
  • Fibrinogen alpha C-chain 5.9 kDa fragment
  • Fibrosis marker
  • Hyaluronic acid
  • Type IV collagen

ASJC Scopus subject areas

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus'. Together they form a unique fingerprint.

  • Cite this

    Sogawa, K., Noda, K., Umemura, H., Seimiya, M., Kuga, T., Tomonaga, T., Nishimura, M., Kanai, F., Imazeki, F., Takizawa, H., Yoneda, M., Nakajima, A., Tsutsumi, M., Yokosuka, O., & Nomura, F. (2013). Serum fibrinogen alpha C-chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus. Proteomics - Clinical Applications, 7(5-6), 424-431. https://doi.org/10.1002/prca.201200094