Serum antibodies to Helicobacter pylori and its heat-shock protein 60 correlate with the response of gastric mucosa-associated lymphoid tissue lymphoma to eradication of H. pylori

Ryuta Takenaka, Kenji Yokota, Motowo Mizuno, Hiroyuki Okada, Tatsuya Toyokawa, Rie Yamasaki, Tadashi Yoshino, Toshiro Sugiyama, Masahiro Asaka, Yasushi Shiratori, Keiji Oguma

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and aims. Eradication of Helicobacter pylori leads to regression of mucosa-associated lymphoid tissue (MALT) lymphomas. In this study, we measured serum antibodies to H. pylori and H. pylori-recombinant heat-shock protein 60 (rHSP60) in patients with gastric MALT lymphoma to determine whether humoral immune responses to the bacterial antigens correlate with the efficacy of eradication therapy. Methods. Serum samples were obtained from 33 patients with H. pylori-positive gastric MALT lymphoma before undergoing therapy to eradicate the bacteria. Anti-H. pylori antibodies were measured in a commercial assay and in immunoassays to lysates and rHSP60 which were prepared from ATCC 43504 strain. Results. Helicobacter pylori were eradicated in all 33 patients, and the lymphoma completely regressed histologically in 26 patients (79%). Pre-treatment titers of serum antibody to H. pylori and to rHSP60 in the patients whose tumor regressed were significantly higher than titers in patients whose tumors did not regress (p = .0011 and .035, respectively). By logistic regression analysis, age (odds ratio = 0.88, 95% confidence interval = 0.80-0.99), endoscopic appearance (0.053, 0.004-0.65), titers of anti-H. pylori antibodies (67.6, 2.5-1800), and titers of anti-rHSP60 antibody (6.4,1.2-36) were identified as significantly associated factors with the outcome of MALT lymphoma. Conclusions. Measurement of serum antibodies to H. pylori and HSP60 might be useful for predicting the response of gastric MALT lymphoma to eradication of H. pylori.

Original languageEnglish
Pages (from-to)194-200
Number of pages7
JournalHelicobacter
Volume9
Issue number3
DOIs
Publication statusPublished - 2004

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Chaperonin 60
Marginal Zone B-Cell Lymphoma
Gastric Mucosa
Helicobacter pylori
Antibodies
Serum
Recombinant Proteins
Bacterial Antigens
Humoral Immunity
Immunoassay
Lymphoma
Neoplasms
Therapeutics
Logistic Models
Odds Ratio
Regression Analysis
Confidence Intervals

Keywords

  • Antibody
  • Heat-shock protein 60
  • Helicobacter pylori
  • MALT lymphoma

ASJC Scopus subject areas

  • Gastroenterology
  • Microbiology

Cite this

Serum antibodies to Helicobacter pylori and its heat-shock protein 60 correlate with the response of gastric mucosa-associated lymphoid tissue lymphoma to eradication of H. pylori. / Takenaka, Ryuta; Yokota, Kenji; Mizuno, Motowo; Okada, Hiroyuki; Toyokawa, Tatsuya; Yamasaki, Rie; Yoshino, Tadashi; Sugiyama, Toshiro; Asaka, Masahiro; Shiratori, Yasushi; Oguma, Keiji.

In: Helicobacter, Vol. 9, No. 3, 2004, p. 194-200.

Research output: Contribution to journalArticle

Takenaka, Ryuta ; Yokota, Kenji ; Mizuno, Motowo ; Okada, Hiroyuki ; Toyokawa, Tatsuya ; Yamasaki, Rie ; Yoshino, Tadashi ; Sugiyama, Toshiro ; Asaka, Masahiro ; Shiratori, Yasushi ; Oguma, Keiji. / Serum antibodies to Helicobacter pylori and its heat-shock protein 60 correlate with the response of gastric mucosa-associated lymphoid tissue lymphoma to eradication of H. pylori. In: Helicobacter. 2004 ; Vol. 9, No. 3. pp. 194-200.
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T1 - Serum antibodies to Helicobacter pylori and its heat-shock protein 60 correlate with the response of gastric mucosa-associated lymphoid tissue lymphoma to eradication of H. pylori

AU - Takenaka, Ryuta

AU - Yokota, Kenji

AU - Mizuno, Motowo

AU - Okada, Hiroyuki

AU - Toyokawa, Tatsuya

AU - Yamasaki, Rie

AU - Yoshino, Tadashi

AU - Sugiyama, Toshiro

AU - Asaka, Masahiro

AU - Shiratori, Yasushi

AU - Oguma, Keiji

PY - 2004

Y1 - 2004

N2 - Background and aims. Eradication of Helicobacter pylori leads to regression of mucosa-associated lymphoid tissue (MALT) lymphomas. In this study, we measured serum antibodies to H. pylori and H. pylori-recombinant heat-shock protein 60 (rHSP60) in patients with gastric MALT lymphoma to determine whether humoral immune responses to the bacterial antigens correlate with the efficacy of eradication therapy. Methods. Serum samples were obtained from 33 patients with H. pylori-positive gastric MALT lymphoma before undergoing therapy to eradicate the bacteria. Anti-H. pylori antibodies were measured in a commercial assay and in immunoassays to lysates and rHSP60 which were prepared from ATCC 43504 strain. Results. Helicobacter pylori were eradicated in all 33 patients, and the lymphoma completely regressed histologically in 26 patients (79%). Pre-treatment titers of serum antibody to H. pylori and to rHSP60 in the patients whose tumor regressed were significantly higher than titers in patients whose tumors did not regress (p = .0011 and .035, respectively). By logistic regression analysis, age (odds ratio = 0.88, 95% confidence interval = 0.80-0.99), endoscopic appearance (0.053, 0.004-0.65), titers of anti-H. pylori antibodies (67.6, 2.5-1800), and titers of anti-rHSP60 antibody (6.4,1.2-36) were identified as significantly associated factors with the outcome of MALT lymphoma. Conclusions. Measurement of serum antibodies to H. pylori and HSP60 might be useful for predicting the response of gastric MALT lymphoma to eradication of H. pylori.

AB - Background and aims. Eradication of Helicobacter pylori leads to regression of mucosa-associated lymphoid tissue (MALT) lymphomas. In this study, we measured serum antibodies to H. pylori and H. pylori-recombinant heat-shock protein 60 (rHSP60) in patients with gastric MALT lymphoma to determine whether humoral immune responses to the bacterial antigens correlate with the efficacy of eradication therapy. Methods. Serum samples were obtained from 33 patients with H. pylori-positive gastric MALT lymphoma before undergoing therapy to eradicate the bacteria. Anti-H. pylori antibodies were measured in a commercial assay and in immunoassays to lysates and rHSP60 which were prepared from ATCC 43504 strain. Results. Helicobacter pylori were eradicated in all 33 patients, and the lymphoma completely regressed histologically in 26 patients (79%). Pre-treatment titers of serum antibody to H. pylori and to rHSP60 in the patients whose tumor regressed were significantly higher than titers in patients whose tumors did not regress (p = .0011 and .035, respectively). By logistic regression analysis, age (odds ratio = 0.88, 95% confidence interval = 0.80-0.99), endoscopic appearance (0.053, 0.004-0.65), titers of anti-H. pylori antibodies (67.6, 2.5-1800), and titers of anti-rHSP60 antibody (6.4,1.2-36) were identified as significantly associated factors with the outcome of MALT lymphoma. Conclusions. Measurement of serum antibodies to H. pylori and HSP60 might be useful for predicting the response of gastric MALT lymphoma to eradication of H. pylori.

KW - Antibody

KW - Heat-shock protein 60

KW - Helicobacter pylori

KW - MALT lymphoma

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DO - 10.1111/j.1083-4389.2004.00225.x

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