Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning

Miho Kaneko, Toshiro Ono, Tomoyo Matsubara, Yukiyo Yamamoto, Hitoshi Ikeda, Takashi Yoshiki, Susumu Furukawa, Eiichi Nakayama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We showed IgG immune response against endothelial antigens in sera obtained from convalescent Kawasaki disease (KD) patients after recovery from the disease during the follow-up period using serological analysis of recombinant cDNA expression library (SEREX) methodology. We identified 46 antigens represented by 69 clones by immunoscreening of a cDNA expression library from tumor necrosis factor-α (TNFα) treated human umbilical vein endothelial cells (HUVEC) with sera from 4 KD patients. They included ubiquitin pathway proteins, transcriptional factors, signal transduction molecules, metabolic enzymes, cytoskeletal proteins, an adhesion molecule, and a cell cycle protein. By serological survey using phage plaque assay, sera from 5 non-KD patients were rarely reactive with the antigens. Among the antigens, tropomyosin was most frequently isolated (18 of 69 clones). Seventeen of the 18 clones were identified using KD3 serum. The second most frequently isolated antigen was also a cytoskeletal protein, called T-plastin (3 of 69 clones).

Original languageEnglish
Pages (from-to)703-711
Number of pages9
JournalMicrobiology and Immunology
Volume48
Issue number9
Publication statusPublished - 2004

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Mucocutaneous Lymph Node Syndrome
Organism Cloning
Antigens
Clone Cells
Cytoskeletal Proteins
Serum
Gene Library
Cell Cycle Proteins
Tropomyosin
Human Umbilical Vein Endothelial Cells
Ubiquitin
Bacteriophages
Signal Transduction
Tumor Necrosis Factor-alpha
Immunoglobulin G
Enzymes
Proteins

Keywords

  • Endothelial antigen
  • IgG antibody
  • Kawasaki disease
  • SEREX

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Kaneko, M., Ono, T., Matsubara, T., Yamamoto, Y., Ikeda, H., Yoshiki, T., ... Nakayama, E. (2004). Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning. Microbiology and Immunology, 48(9), 703-711.

Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning. / Kaneko, Miho; Ono, Toshiro; Matsubara, Tomoyo; Yamamoto, Yukiyo; Ikeda, Hitoshi; Yoshiki, Takashi; Furukawa, Susumu; Nakayama, Eiichi.

In: Microbiology and Immunology, Vol. 48, No. 9, 2004, p. 703-711.

Research output: Contribution to journalArticle

Kaneko, M, Ono, T, Matsubara, T, Yamamoto, Y, Ikeda, H, Yoshiki, T, Furukawa, S & Nakayama, E 2004, 'Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning', Microbiology and Immunology, vol. 48, no. 9, pp. 703-711.
Kaneko, Miho ; Ono, Toshiro ; Matsubara, Tomoyo ; Yamamoto, Yukiyo ; Ikeda, Hitoshi ; Yoshiki, Takashi ; Furukawa, Susumu ; Nakayama, Eiichi. / Serological identification of endothelial antigens predominantly recognized in Kawasaki disease patients by recombinant expression cloning. In: Microbiology and Immunology. 2004 ; Vol. 48, No. 9. pp. 703-711.
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