Serological analysis of BALB/c methylcholanthrene sarcoma Meth A by SEREX: Identification of a cancer/testis antigen

Toshiro Ono, Shuichiro Sato, Nobuhiko Kimura, Motoyuki Tanaka, Akira Shibuya, Lloyd J. Old, Eiichi Nakayama

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26 Citations (Scopus)

Abstract

Antigens of BALB/c methylcholanthrene-induced fibrosarcoma Meth A recognized by the host humoral immune response were investigated by serological analysis of antigens by recombinant expression cloning (SEREX). Immunoscreening a cDNA library from Meth A (Kγ) cells (Meth A retrovirally transfected with murine IFN-γ cDNA) with sera from BALB/c mice growing parental Meth A transplants identified 10 antigens. One of them, OY-MS-4, showed characteristics of a cancer/testis (CT) antigen. Nucleotide sequence analysis revealed that OY-MS-4 was identical to a mouse placenta and embryonic expression gene (pem) known to be selectively expressed during embryogenesis and in transformed cell lines. In adult mice, expression of OY-MS-4 was restricted to testis and placenta. Four of 6 methylcholanthrene-induced fibrosarcomas in BALB/c mice showed strong expression of OY-MS-4. In 6 T-cell leukemias, only a dimethylbenzanthracene-induced leukemia, EL4. (C57BL), showed strong expression. Two other tumors, A20.2J and P815, induced by ethylnitrosourea and methylcholanthrene, respectively, also strongly expressed OY-MS-4. The other 9 gene products identified in Meth A by SEREX were expressed in all 15 tumors tested and in a range of normal tissues. Sequence analysis of cDNA inserts coding for the SEREX-defined antigens showed no evidence of mutation. Despite the expression of OY-MS-I-10 antigens in methylcholanthrene sarcomas other than Meth A, no antibody was detected in the sera of mice bearing these other sarcomas. The basis for the unique immunogenicity of OY-MS-I-10 presented by Meth A, but not by other syngeneic tumors expressing these gene products, is unknown. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalInternational Journal of Cancer
Volume88
Issue number6
DOIs
Publication statusPublished - Dec 15 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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